Equipment Equipment

The Chemical Analysis Suite uses a number of analytical techniques including Gas Chromatography, Liquid Chromatography and Colourimetry.

The Chemical Analysis Laboratory experienced technical staff is able to provide an analytical service in handling samples from the atmosphere, geosphere, hydrosphere and industry.

This service is available to the University Of East Anglia (UEA), the Norwich Research Park (NRP) and the wider scientific and business community in the UK and abroad. We offer a complete analytical package, from sampling and preparing to analysing and reporting results.

When required we can train personnel in the analytical techniques associated with their samples, including preparation and instrument control.  Once trained, users can book the instruments to carry out their own research and analysis.

All enquiries are also welcome and should initially be addressed to Liz Rix, Facilities Manager E.Rix@uea.ac.uk 01603 592540

We have the following equipment available for use

Skalar San++ Flow Analyser

The instrument has the ability to analyse samples in different matrices, i.e. fresh water, seawater & extractions in a salt solution. The wash solution that carries the samples through the instrument can be matrix matched to the samples. The minimum volume required to determine the concentration of each nutrient (channel) is 1.5mL, thus to determine Nitrite, Ammonia/Ammonium and Silicate a minimum of 4.5mL is required.

Analysis is done using selective chemical reagents that are administered at a prescribed rate to produce a coloured compound. The intensity of the colour is proportional to the nutrient concentration in the sample, which is measured by a fixed wavelength spectrophotometer connected to each channel.

Perkin Elmer Clarus 500 with HS-40 GC

The system is configured to simultaneously measure Nitrous Oxide, Carbon Dioxide and Methane gas, from either air or liquid samples (dissolved gas).

We measure these using a TurboMatrix HS-40 automated headspace autosampler system coupled to the Clarus 500 GC containing both ECD and FID detectors.

Dionex ICS2000

The Dionex ion chromatography system ICS-2000 allows the quantitative analysis of negatively charged ions (anions) in aqueous solutions using suppressed conductivity detection.

Ion chromatography is a form of liquid chromatography that uses ion-exchange resins to separate ions and polar molecules based on their interaction with the resin and the equilibrium distribution of the sample components between two different phases – the mobile phase and the stationary phase. The mobile phase is the eluent and the stationary phase is the ion exchange resin within the chromatography column. The sample components migrate through the system when they are in the mobile phase, and their velocity is a function of equilibrium distribution. The components that have distributions which favour the stationary phase migrate slower than those which have distributions favouring the mobile phase.

Dionex ICS5000

The Dionex ion chromatography system ICS-5000 allows the quantitative analysis of negatively charged ions (anions) and positively charged ions (cations) in aqueous solutions using suppressed conductivity detection.

Ion chromatography is a form of liquid chromatography that uses ion-exchange resins to separate ions and polar molecules based on their interaction with the resin and the equilibrium distribution of the sample components between two different phases – the mobile phase and the stationary phase. The mobile phase is the eluent and the stationary phase is the ion exchange resin within the chromatography column. The sample components migrate through the system when they are in the mobile phase, and their velocity is a function of equilibrium distribution. The components that have distributions which favour the stationary phase migrate slower than those which have distributions favouring the mobile phase.

Agilent 7820A Gas Chromatograph

Agilent 7820A Gas Chromatograph with Flame Ionization Detector and 16 place autosampler.

The instrument can be fitted with a DB-5, HP-5 or Supelcowax 10 depending on the application.

Gas chromatography is an analytical technique that separates and detects components within a volatile mixture. It uses a gaseous mobile phase, or carrier gas, to transport sample components through a capillary column containing a polymeric stationary phase. The samples are separated according to their affinity for the stationary phase and relative vapour pressure.

On elution from the column, the analytes are detected by a flame ionisation detector which produces an electronic signal and generates a chromatogram from which analytes can be identified according to their retention times.

Dionex HPLC UltiMate 3000

Dionex HPLC UltiMate 3000 is a high pressure liquid chromatography system with a photo diode array detector and a fluorescence detector.

HPLC is used to separate, identify and quantify chemical compounds in liquid samples. This involves a liquid sample being passed over a solid adsorbent material packed into a column using a flow of liquid solvent. Each analyte in the sample interacts slightly differently with the adsorbent material, thus retarding the flow of the analytes. If the interaction is weak, the analytes flow off the column in a short amount of time, and if the interaction is strong, then the elution time is long.

 

Perkin Elmer Clarus 500 GCMS

Perkin Elmer Clarus 500 gas chromatograph coupled to a Clarus 500 mass spectrometer complete with liquid autosampler