Biography

Leanne obtained a first class BSc (Dual Honours) in Physiology & Pharmacology from the University of Sheffield in 1999 and continued to study an MRes (MPhil) in Immunology & oncology at the University of Birmingham (2000). She stayed at the University of Birmingham to do a PhD in Immunology under the supervision of Professor John Gordon and Dr Gillian Grafton.

Following her interests in ion channels in immune cells, Leanne began a post-doctoral position in the lab of Professor Annmarie Surprenant at the University of Sheffield (2003-2006) investigating purinergic ion channels. A further post-doctoral position at the University of Sydney with Professor James Wiley (2007-2010) led to independence as a research fellow at the University of Sydney (2010-2013) followed by a prestigious Vice Chancellor’s research fellowship at RMIT University in Melbourne (2013-2015). 

Selected publications

Helliwell RM, ShioukHuey C O, Dhuna K, Molero JC, Ye J-M, Xue CC, Stokes L.
Selected ginsenosides of the protopanaxdiol series are novel positive allosteric modulators of P2X7 receptors.
Br J Pharm. 2015 Epub ahead
DOI: 10.1111/bph.13123.

Bartlett R, Stokes L, Sluyter R.
The P2X7 purinergic receptor: recent developments and the use of P2X7 antagonists in models of disease.
Pharmacological Reviews 2014 66(3):638-75.
DOI: 10.1124/pr.113.008003.

Bhaskaracharya A, Dao-Ung P, Spildrejorde M, Jalilian I, Skarratt KK, Fuller SJ, Sluyter R, Stokes L.
Probenecid blocks P2X7 receptor-induced dye uptake via a pannexin-1 independent mechanism.
PLoS One 2014 9(3):e93058.
DOI: 10.1371/journal.pone.0093058

Stokes L, Scurrah K, Ellis JA, Cromer BA, Skarratt KK, Gu BJ, Harrap SB, Wiley JS.
A loss-of-function polymorphism in the human P2X4 receptor is associated with increased pulse pressure.
Hypertension 2011 58(6):1086-92.
DOI: 10.1161/HYPERTENSIONAHA.111.176180

Stokes L, Fuller SJ, Sluyter R, Skarratt KK, Gu B, Wiley JS.
Two haplotypes of the P2X7 receptor containing the Ala-348 to Thr polymorphism exhibit a gain-of-function effect and enhanced interleukin-1b secretion.
FASEB Journal. 2010 24(8): 2916-27.
DOI: 10.1096/fj.09-150862

All Publications

Mills, S., Howell, L., Beekman, A., Stokes, L., Mueller, A.

(2018)

Rac1 plays a role in CXCL12 but not CCL3-induced chemotaxis and Rac1 GEF inhibitor NSC23766 has off target effects on CXCR4,

in Cellular Signalling

42

pp. 88–96

Full Text UEA Repository

(Article)

(Published)


Wilkinson, S. M., Barron, M. L., O'Brien-Brown, J., Janssen, B., Stokes, L., Werry, E. L., Chishty, M., Skarratt, K. K., Ong, J. A., Hibbs, D. E., Vugts, D. J., Fuller, S., Windhorst, A. D., Kassiou, M.

(2017)

Pharmacological Evaluation of Novel Bioisosteres of an Adamantanyl Benzamide P2X7 Receptor Antagonist,

in ACS Chemical Neuroscience

8

(11)

pp. 2374–2380

Full Text UEA Repository

(Article)

(Published)


De Luca, S. N., Ziko, I., Dhuna, K., Sominsky, L., Tolcos, M., Stokes, L., Spencer, S. J.

(2017)

Neonatal overfeeding by small-litter rearing sensitises hippocampal microglial responses to immune challenge: Reversal with neonatal repeated injections of saline or minocycline,

in Journal of Neuroendocrinology

29

(11)

article no. e12540

Full Text UEA Repository

(Article)

(Published)


Stokes, L., Layhadi, J. A., Bibic, L., Dhuna, K., Fountain, S. J.

(2017)

P2X4 receptor function in the nervous system and current breakthroughs in pharmacology,

in Frontiers in Pharmacology

8

article no. 291

Full Text UEA Repository

(Article)

(Published)


Gilbert, D. F., Stebbing, M. J., Kuenzel, K., Murphy, R. M., Zacharewicz, E., Buttgereit, A., Stokes, L., Adams, D. J., Friedrich, O.

(2016)

Store-Operated Ca2+ Entry (SOCE) and Purinergic Receptor-Mediated Ca2+ Homeostasis in Murine bv2 Microglia Cells: Early Cellular Responses to ATP-Mediated Microglia Activation,

in Frontiers in Molecular Neuroscience

9

article no. 111

Full Text UEA Repository

(Article)

(Published)


Stokes, L., Mackenzie, A., Sluyter, R.

(2016)

Roles of ion channels in immune cells,

in Frontiers in Immunology

48

article no. 7

Full Text

(Editorial)

(Published)


Dao-Ung, P., Skarratt, K., Fuller, S., Stokes, L.

(2015)

Paroxetine suppresses recombinant human P2X7 responses,

in Purinergic Signalling

11

(4)

pp. 481-490

Full Text UEA Repository

(Article)

(Published)


Stokes, L., Spencer, S. J., Jenkins, T. A.

(2015)

Understanding the role of P2X7 in affective disorders—are glial cells the major players?,

in Frontiers in Cellular Neuroscience

9

article no. 258

Full Text UEA Repository

(Article)

(Published)


Helliwell, R., ShioukHuey, C., Dhuna, K., Molero, J., Ye, J., Xue, C., Stokes, L.

(2015)

Selected ginsenosides of the prptopanaxdiol series are novel positive allosteric modulators of P2X7 receptors,

in British Journal of Pharmacology

172

(13)

pp. 3326-3340

Full Text UEA Repository

(Article)

(Published)


Ziko, I., De Luca, S. N., Dinan, T., Barwood, J. M., Sominsky, L., Cai, G., Kenny, R., Stokes, L., Jenkins, T. A., Spencer, S. J.

(2014)

Neonatal overfeeding alters hypothalamic microglial profiles and central responses to immune challenge long-term,

in Brain, Behavior, and Immunity

41

pp. 32-43

Full Text UEA Repository

(Article)

(Published)


Higgins, K. R., Kovacevic, W., Stokes, L.

(2014)

Nucleotides Regulate Secretion of the Inflammatory Chemokine CCL2 from Human Macrophages and Monocytes,

in Mediators of Inflammation

2014

article no. 293925

Full Text UEA Repository

(Article)

(Published)


Bhaskaracharya, A., Dao-Ung, P., Jalilian, I., Spildrejorde, M., Skarratt, K. K., Fuller, S. J., Sluyter, R., Stokes, L.

(2014)

Probenecid Blocks Human P2X7 Receptor-Induced Dye Uptake via a Pannexin-1 Independent Mechanism,

in PLoS ONE

9

(3)

article no. e93058

Full Text UEA Repository

(Article)

(Published)


Key Research Interests

I am interested in the roles ion channels play in the regulation of immune cell signalling. My focus is on the purinergic P2X channel family which are activated by extracellular ATP, in particular P2X7. This ion channel has been implicated in a number of inflammatory disorders and pharmacological interventions may be useful in the design of future therapies.

Current projects involve studying natural products for effect on P2X channels including compounds from traditional chinese medicines. I am also interested in the effect natural genetic mutations or polymorphisms can have on ion channels and have spent several years investigating impact of SNPs on P2X7 and P2X4 channel functions.