Leanne obtained a first class BSc (Dual Honours) in Physiology & Pharmacology from the University of Sheffield in 1999 and continued to study an MRes (MPhil) in Immunology & oncology at the University of Birmingham (2000). She stayed at the University of Birmingham to do a PhD in Immunology under the supervision of Professor John Gordon and Dr Gillian Grafton.
Following her interests in ion channels in immune cells, Leanne began a post-doctoral position in the lab of Professor Annmarie Surprenant at the University of Sheffield (2003-2006) investigating purinergic ion channels. A further post-doctoral position at the University of Sydney with Professor James Wiley (2007-2010) led to independence as a research fellow at the University of Sydney (2010-2013) followed by a prestigious Vice Chancellor’s research fellowship at RMIT University in Melbourne (2013-2015).
Helliwell RM, ShioukHuey C O, Dhuna K, Molero JC, Ye J-M, Xue CC, Stokes L.
Selected ginsenosides of the protopanaxdiol series are novel positive allosteric modulators of P2X7 receptors.
Br J Pharm. 2015 Epub ahead
Bartlett R, Stokes L, Sluyter R.
The P2X7 purinergic receptor: recent developments and the use of P2X7 antagonists in models of disease.
Pharmacological Reviews 2014 66(3):638-75.
Bhaskaracharya A, Dao-Ung P, Spildrejorde M, Jalilian I, Skarratt KK, Fuller SJ, Sluyter R, Stokes L.
Probenecid blocks P2X7 receptor-induced dye uptake via a pannexin-1 independent mechanism.
PLoS One 2014 9(3):e93058.
Stokes L, Scurrah K, Ellis JA, Cromer BA, Skarratt KK, Gu BJ, Harrap SB, Wiley JS.
A loss-of-function polymorphism in the human P2X4 receptor is associated with increased pulse pressure.
Hypertension 2011 58(6):1086-92.
Stokes L, Fuller SJ, Sluyter R, Skarratt KK, Gu B, Wiley JS.
Two haplotypes of the P2X7 receptor containing the Ala-348 to Thr polymorphism exhibit a gain-of-function effect and enhanced interleukin-1b secretion.
FASEB Journal. 2010 24(8): 2916-27.
Rac1 plays a role in CXCL12 but not CCL3-induced chemotaxis and Rac1 GEF inhibitor NSC23766 has off target effects on CXCR4,
in Cellular Signalling
pp. 88–96Full Text UEA Repository
Pharmacological Evaluation of Novel Bioisosteres of an Adamantanyl Benzamide P2X7 Receptor Antagonist,
in ACS Chemical Neuroscience
pp. 2374–2380Full Text UEA Repository
Neonatal overfeeding by small-litter rearing sensitises hippocampal microglial responses to immune challenge: Reversal with neonatal repeated injections of saline or minocycline,
in Journal of Neuroendocrinology
article no. e12540Full Text UEA Repository
P2X4 receptor function in the nervous system and current breakthroughs in pharmacology,
in Frontiers in Pharmacology
article no. 291Full Text UEA Repository
Store-Operated Ca2+ Entry (SOCE) and Purinergic Receptor-Mediated Ca2+ Homeostasis in Murine bv2 Microglia Cells: Early Cellular Responses to ATP-Mediated Microglia Activation,
in Frontiers in Molecular Neuroscience
article no. 111Full Text UEA Repository
Roles of ion channels in immune cells,
in Frontiers in Immunology
article no. 7Full Text
Paroxetine suppresses recombinant human P2X7 responses,
in Purinergic Signalling
pp. 481-490Full Text UEA Repository
Understanding the role of P2X7 in affective disorders—are glial cells the major players?,
in Frontiers in Cellular Neuroscience
article no. 258Full Text UEA Repository
Selected ginsenosides of the prptopanaxdiol series are novel positive allosteric modulators of P2X7 receptors,
in British Journal of Pharmacology
pp. 3326-3340Full Text UEA Repository
Neonatal overfeeding alters hypothalamic microglial profiles and central responses to immune challenge long-term,
in Brain, Behavior, and Immunity
pp. 32-43Full Text UEA Repository
Nucleotides Regulate Secretion of the Inflammatory Chemokine CCL2 from Human Macrophages and Monocytes,
in Mediators of Inflammation
article no. 293925Full Text UEA Repository
Probenecid Blocks Human P2X7 Receptor-Induced Dye Uptake via a Pannexin-1 Independent Mechanism,
in PLoS ONE
article no. e93058Full Text UEA Repository
Key Research Interests
I am interested in the roles ion channels play in the regulation of immune cell signalling. My focus is on the purinergic P2X channel family which are activated by extracellular ATP, in particular P2X7. This ion channel has been implicated in a number of inflammatory disorders and pharmacological interventions may be useful in the design of future therapies.
Current projects involve studying natural products for effect on P2X channels including compounds from traditional chinese medicines. I am also interested in the effect natural genetic mutations or polymorphisms can have on ion channels and have spent several years investigating impact of SNPs on P2X7 and P2X4 channel functions.