Despite the harsh environmental conditions in polar oceans, these ecosystems harbour significant biodiversity. How this biodiversity underpinning productive ecosystems and resources for novel products used in biotechnology, agriculture and medicine has evolved largely remains unknown. One reason for this knowledge gap is that we only know little as to how mutation and recombination as major evolutionary forces shape polar organisms and therefore their genes enabling them to thrive under the harsh polar growth conditions. Arguably, most of the genetic variation driving biological diversity stems from mutations. As their rate is temperature-dependent with increasing mutation rates at the extreme ends, it suggests that polar organisms likely need to cope with higher mutational loads. Polar diatom genomes have provided evidence that these microalgae benefit from elevated mutation rates, generating genetic diversity to respond to fast-changing environmental conditions (e.g. freezing and melting) in the absence of sexual recombination. However, direct experimental evidence is missing. Thus, this PhD studentship will investigate if DNA damage is enhanced in a polar diatom vs a non-polar counterpart and how mitotic recombination contributes to the genetic variation that is adaptive. The student will use fluorescence-based assays in combination with genome re-sequencing to quantify DNA damage after cold and heat stress in a polar and a nonpolar diatom species. To test how DNA-repair induced mitotic recombination contributes to genetic variation and differences between both diatom species, the student will knock out two core DNA-repair enzymes (BRCA2, KU70) and will reveal subsequent effects on DNA damage and fitness (Growth rates) under temperature stress. Thus, data from this project will lay the foundation for how the extreme polar environment shapes the evolution of genes and genomes in polar phytoplankton underpinning productive food webs and resources for novel enzymes used in biotechnology.
The Norwich Research Park Biosciences Doctoral Training Partnership (NRPDTP) is open to the UK and international candidates for entry in October 2022 and offers postgraduates the opportunity to undertake a 4-year PhD research project whilst enhancing professional development and research skills through a comprehensive training programme. You will join a vibrant community of world-leading researchers. All NRPDTP students undertake a three-month professional internship placement (PIPS) during their study. The placement offers exciting and invaluable work experience designed to enhance professional development. Full support and advice will be provided by our Professional Internship team. Students with, or expecting to attain, at least an upper second class honours degree, or equivalent, are invited to apply.
This project has been shortlisted for funding by the NRPDTP programme. Shortlisted applicants will be interviewed on Tuesday 25th January, Wednesday 26th January and Thursday 27th January 2022.
You can visit the Norwich Research Park Biosciences Doctoral Training Partnership pages for further information on eligibility and the application process.
Our partners value diverse and inclusive work environments that are positive and supportive. Students are selected for admission without regard to gender, marital or civil partnership status, disability, race, nationality, ethnic origin, religion or belief, sexual orientation, age or social background.