Biography

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Tharin obtained a BSc in Molecular Sciences and a PhD in Biochemistry from the University of São Paulo (Brazil). Her interests in Structural Biology then took her to Canada, where she used nuclear magnetic resonance (NMR) spectroscopy to study protein structure and dynamics in the regulation of muscle contraction as a postdoctoral fellow in Prof. Brian Sykes’s laboratory, in the University of Alberta, before moving to the UK to work with Prof. Steve Matthews at Imperial College London, where she studied proteins involved in cell invasion by the parasite Toxoplasma gondii.

Research interests in the Blumenschein group involve using NMR spectroscopy to investigate structural and dynamic properties of proteins, and their role in protein interactions and function. Current projects include Chlamydia effectors, cell signalling in cancer, and the cytochrome c, as well as the study of intrinsically disordered proteins by NMR spectroscopy.

Selected publications

A. I. Karsisiotis, O. M. Deacon, M. T. Wilson, C. Macdonald, T. M. A. Blumenschein, G. R. Moore and J. R. Worrall.
Increased dynamics in the 40-57 Ω-loop of the G41S variant of human cytochrome c promote its pro-apoptotic conformation.
Scientific Reports, 2016, 6, 30447.
DOI:10.1038/srep30447

L. S. Boutemy, S. R. King, J. Win, R. K. Hughes, T. A. Clarke, T. M. A. Blumenschein, S. Kamoun and M. J. Banfield.
Structures of Phytophthora RXLR effector proteins: a conserved but adaptable fold underpins functional diversity.
Journal of Biological Chemistry, 2011, 286, 35834-35842.
DOI: 10.1074/jbc.M111.262303

J. F. de Oliveira, M. L. Sforça, T. M. A. Blumenschein, B. G. Guimarães, C. C. de Oliveira, N. I. T. Zanchin and A.-C. Zeri.
Structure, dynamics and RNA interaction analysis of the human SBDS protein
Journal of Molecular Biology, 2010, 396, 1053-1069.
DOI: 10.1016/j.jmb.2009.12.039

T. M. A. Blumenschein, N. Friedrich, R. A. Childs, S. Saouros, E. P. Carpenter, M. A. Campanero-Rhodes, P. Simpson, W. Chai, T. Koutroukides, M. J. Blackman, T. Feizi, D. Soldati-Favre and S. Matthews.
Atomic resolution insight into host cell recognition by Toxoplasma gondii.
EMBO Journal, 2007, 26, 2808-2820.
DOI: 10.1038/sj.emboj.7601704

Career History

  • 1990-1994 – BSc in Molecular Sciences, University of São Paulo, Brazil
  • 1995-2000 – PhD in Biochemistry, Institute of Chemistry, University of São Paulo, Brazil 
  • 2000-2005 – Postdoctoral Fellow, Department of Biochemistry, University of Alberta, Canada
  • 2005-2007 – Postdoctoral Research Associate, Division of Molecular Biosciences, Imperial College London
  • 2007-current – Lecturer in Biomolecular NMR, School of Chemistry, University of East Anglia

All Publications

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Karsisiotis, A. I., Deacon, O. M., Wilson, M. T., Macdonald, C., Blumenschein, T. M., Moore, G. R., Worrall, J. R.

(2016)

Increased dynamics in the 40-57 Ω-loop of the G41S variant of human cytochrome c promote its pro-apoptotic conformation

in Scientific Reports

6.

Full Text UEA Repository

(Article)


Karsisiotis, A. I., Deacon, O. M., Rajagopal, B., Macdonald, C., Blumenschein, T. M. A., Moore, G., Worrall, J. R.

(2015)

Backbone resonance assignments of ferric human cytochrome c and the pro-apoptotic G41S mutant in the ferric and ferrous states

in Biomolecular NMR Assignments

9.

pp. 415-419

Full Text UEA Repository

(Article)


Boutemy, L., King, S., Win, J., Hughes, R., Clarke, T., Blumenschein, T., Kamoun, S., Banfield, M.

(2011)

Structures of Phytophthora RXLR Effector Proteins: a CONSERVED BUT ADAPTABLE FOLD UNDERPINS FUNCTIONAL DIVERSITY

in Journal of Biological Chemistry

286.

pp. 35834-35842

Full Text UEA Repository

(Article)


Julien, O., Mercier, P., Allen, C. N., Fisette, O., Ramos, C. H. I., Lagüe, P., Blumenschein, T. M. A., Sykes, B. D.

(2011)

Is there nascent structure in the intrinsically disordered region of troponin I?

in Proteins: Structure, Function, and Bioinformatics

79.

pp. 1240-1250

Full Text UEA Repository

(Article)


de Oliveira, J. F., Sforça, M. L., Blumenschein, T., Goldfeder, M. B., Guimarães, B. G., Oliveira, C. C., Zanchin, N. I., Zeri, A.

(2010)

Structure, Dynamics, and RNA Interaction Analysis of the Human SBDS Protein

in Journal of Molecular Biology

396.

pp. 1053-1069

Full Text UEA Repository

(Article)


Blumenschein, T.

(2009)

Biomolecular structures by solution magnetic nuclear resonance

Wiley-VHC Verlag GmbH & Co KGaA, Weinheim

pp. 963-987

ISBN 978-3-527-40773-6

UEA Repository

(Chapter)


Blumenschein, T., Friedrich, N., Childs, R. A., Saouros, S., Carpenter, E. P., Campanero-Rhodes, M. A., Simpson, P., Chai, W., Koutroukides, T., Blackman, M. J., Feizi, T., Soldati-Favre, D., Matthews, S.

(2007)

Atomic resolution insight into host cell recognition by Toxoplasma gondii

in The EMBO Journal

26.

pp. 2808-2820

Full Text UEA Repository

(Article)


Saouros, S., Blumenschein, T., Sawmynaden, K., Marchant, J., Koutroukides, T., Liu, B., Simpson, P., Carpenter, E. P., Matthews, S. J.

(2007)

High-Level Bacterial Expression and Purification of Apicomplexan Micronemal Proteins for Structural Studies

in Protein and Peptide Letters

14.

pp. 411-415

Full Text UEA Repository

(Article)


Blumenschein, T. M. A., Carpenter, E. P., Saouros, S., Friedrich, N., Koutroukides, T., Soldati, D., Matthews, S.

(2007)

The cell-binding region of Toxoplasma gondii micronemal protein 1 presents a novel fold

in Biophysical Journal

pp. 43A

UEA Repository

(Article)


Hoffman, R. M. B., Gagne, S. M., Blumenschein, T. M. A., Sykes, B. D.

(2007)

A functional role for the intrinsically disordered regions of troponin I

in Biophysical Journal

pp. 396A

UEA Repository

(Article)


Blumenschein, T., Stone, D., Fletterick, R., Mendelson, R., Sykes, B.

(2006)

Dynamics of the C-Terminal Region of TnI in the Troponin Complex in Solution

in Biophysical Journal

90.

pp. 2436-2444

Full Text UEA Repository

(Article)


Hoffman, R. M., Blumenschein, T., Sykes, B. D.

(2006)

An Interplay between Protein Disorder and Structure Confers the Ca2+ Regulation of Striated Muscle

in Journal of Molecular Biology

361.

pp. 625-633

Full Text UEA Repository

(Article)


Blumenschein, T., Stone, D., Fletterick, R., Mendelson, A., Sykes, B.

(2005)

Calcium-dependent Changes in the Flexibility of the Regulatory Domain of Troponin C in the Troponin Complex

in The Journal of Biological Chemistry

280.

pp. 21924-21932

Full Text UEA Repository

(Article)


Blumenschein, T. M. A., Stone, D. B., Sykes, B. D.

(2005)

Dynamics of TnC in the skeletal troponin complex

in Biophysical Journal

88.

pp. 359A

UEA Repository

(Article)


Blumenschein, T., Gillis, T. E., Tibbits, G. F., Sykes, B. D.

(2004)

Effect of Temperature on the Structure of Trout Troponin C

in Biochemistry

43.

pp. 4955-4963

Full Text UEA Repository

(Article)


Blumenschein, T. M. A., Stone, D. B., Sykes, B. D.

(2004)

Troponin C in the skeletal ternary complex

in Biophysical Journal

86.

pp. 215A

UEA Repository

(Article)


Blumenschein, T. M. A., Gillis, T. E., Tibbits, G. F., Sykes, B. D.

(2003)

Effects of temperature on protein structure: Trout troponin C

in Biophysical Journal

84.

pp. 567A

UEA Repository

(Article)


Gillis, T. E., Blumenschein, T., Sykes, B. D., Tibbits, G. F.

(2003)

Effect of Temperature and the F27W Mutation on the Ca2+Activated Structural Transition of Trout Cardiac Troponin C

in Biochemistry

42.

pp. 6418-6426

Full Text UEA Repository

(Article)


Blumenschein, T. M. A., Gillis, T. E., Tibbits, G. F., Sykes, B. D.

(2002)

Effect of temperature on trout cardiac troponin C structure

in Biophysical Journal

82.

pp. 1887

UEA Repository

(Article)


Gillis, T. E., Blumenschein, T. M. A., Sykes, B. D., Tibbits, G. F.

(2002)

Site I binds Ca2+ in trout cardiac troponin C

in Biophysical Journal

82.

pp. 1886

UEA Repository

(Article)


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Key Research Interests

Research interests in the Blumenschein group revolve around understanding protein structures and dynamics, and their role in protein interactions and function. We perform protein expression and purification to obtain isotopically labelled samples for nuclear magnetic resonance (NMR) spectroscopy studies, complemented by other biophysical techniques as necessary.

Current research projects include the study of pathogen effectors, cell signalling in cancer and apoptosis, with a particular interest in intrinsically disordered proteins in these areas. Effectors are proteins produced by pathogens that contribute to the process of infection, both of animal and plant hosts. We are currently studying the structure and dynamics of Tarp, an effector from Chlamydia trachomatis involved in the cell invasion in human infection (in collaboration with Dr Ted Hackstadt, NIH, USA).

Cell signalling is intrinsic to the process of cancer. The process of cell division and growth is usually tightly regulated in each cell, involving a number of proteins that interact with each other to maintain this balanced network of signals. We are looking at three different signalling processes that are involved in cancer and apoptosis: ADAM-15 interactions with different intracellular kinases (in collaboration with Prof Dylan Edwards), the E3 ligase WWP2 interactions with its targets (in collaboration with Dr Andrew Chantry), and the role of cytochrome c in apoptosis (in collaboration with Dr Jonathan Worral, Essex).
 

Research Group Membership

Photo of the group enjoying a meal out

The group at the CCPN meeting, summer 2012. From the left: James Tolchard, Vicki Chambers, Krissy Kay, Liz Shedden and Tharin Blumenschein.

PhD Students
Danielle de Bourcier

Summer intern
Hans Pfalzgraf

Former group members
Liz Shedden(PhD student 2011-15)
James Tolchard (PhD student 2009-2014)
Jacob Pullin (summer intern 2015 and undergraduate project student 2015/16)
Jack Dwyer (undergraduate project students 2015/16)
Eleanor Nash (undergraduate project student 2015/16)
Oluchi Opara (undergraduate project student 2015/16)
Sam Walpole (undergraduate summer student 2013 and project student 2014/15)
Jessica Spencer (undergraduate project student 2014/15)
Alex Smith (undergraduate summer student 2014)
Monica Sharman (undergraduate project student 2013/14)
Priscilla Sieira Chaves (undergraduate summer student 2012)
Michael Wood (undergraduate summer student 2012)
Krissy Kay (visiting researcher 2010/11 and MSc project student 2011/12)
Vicki Chambers (undergraduate project student 2011/12)
Alex Carey-Hulyer (undergraduate summer student 2010 and project student 2011/12)
Rachael Gathercole (undergraduate summer student 2011)
Francesca Kinsey (undergraduate summer student 2011)
Claire Allen (undergraduate summer student 2009 and project student 2010/11)
Aaron Eveleigh (undergraduate project student 2009/10)
Lawrence Eaglen (undergraduate project student 2008/09)
Leah Morris (undergraduate summer student 2008)

External Activities and Indicators of Esteem

  • Secretary of the British Biophysical Society Committee since 2013
  • Member of the Royal Society of Chemistry East Anglia Section Committee since 2007
  • Member of the Biochemical Society since 2005
  • Member of the Biophysical Society since 1998

Key Responsibilities

  • Employability Director in the School of Chemistry
  • Science without Borders Coordinator for Chemistry
  • Member of the School of Chemistry Web Committee
  • Member of Natural Sciences extenuating circumstances panel
  • Reserve member of the Faculty Appeals and Complaints panel

Administrative Posts

  • Senior Adviser for Natural Sciences
  • Director of Employability in the School of Chemistry
  • Science without Borders Coordinator for Chemistry
  • Member of the Natural Sciences Teaching & Admissions Executive Team
  • Member of the School of Chemistry Web Committee