Biography

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Myles Cheesman graduated from University of Cambridge with a B.A. in Natural Sciences in 1983. He then moved to UEA to join the group of Professor Andrew Thomson and studied for a PhD on the spectroscopic properties of nickel ions and iron-sulphur clusters in bacterial proteins. Post-doctoral research on heme-copper oxidases was followed by a period as Senior Research Officer responsible for the Magnetic Circular Dichroism (MCD) and Electron Paramagnetic Resonance (EPR) laboratories of the Centre for Metalloprotein Spectroscopy and Biology (CMSB) and then appointment to faculty in 2004.

Myles’ research centres on the use of of MCD and EPR spectroscopy to study proteins containing transition metal ions:

One third of all proteins would not function were it not for the presence of one or more metal cofactors. Enzymes which catalyse the redox transformation of small inorganic substrates, such as O2, NO, NO2- and S2O32-, invariably contain transition metal ions, both as electron transfer centres and at the active site itself. Thus the bacterial enzymes which drive the global nitrogen and sulphur cycles and the enzymes of the O2-terminated respiratory chains of many organisms are almost all transition metalloproteins.

The techniques of MCD and EPR detect species which possess colour and paramagnetism and are therefore eminently suited to the study of these protein-bound inorganic complexes, providing information on metal identity, redox state, nature of ligands and interactions with other adjacent metal sites. These methods are currently used to study a wide variety of metalloproteins with a current emphasis on multiheme enzymes. Much of this work necessitates active collaboration with other research groups within the Henry Wellcome Laboratories for Biological Chemistry and with members of the School of Biological Sciences through the Centre for Metalloprotein Spectroscopy and Biology (CMSB).

Selected Publications

R.S. Pitcher, M.R. Cheesman and N.J.Watmough.
Molecular and spectroscopic analysis of the cytochrome cbb3 oxidase from Pseudomonas stutzeri
J. Biol. Chem., 2002, 277, 31474 – 31483.
DOI: 10.1074/jbc.M204103200

R.S. Zajicek, M.R. Cheesman, E.H.J. Gordon and S.J. Ferguson.
Y25S Variant of Paracoccus pantotrophus Cytochrome cd1 Provides Insight into Anion Binding by d1 Heme and a Rare Example of a Critical Difference between Solution of Crystal Structures
J. Biol Chem., 2005, 280, 26073-26079.
DOI: 10.1074/jbc.M501890200

S.J. Field, L. Prior, M.D. Roldan, M.R. Cheesman, A.J. Thomson, S. Spiro, J.N. Butt, N.J. Watmough and D.J. Richardson.
Spectral properties of bacterial nitric-oxide reductase - Resolution of pH-dependent forms of the active site heme b3
J. Biol. Chem., 2002, 277, 20146 – 20150.
DOI: 10.1074/jbc.M112202200

M.R. Cheesman, P.J. Little, and B.C. Berks.
Novel heme ligation in a c-type cytochrome involved in thiosulfate oxidation: EPR and MCD of SoxAX from Rhodovulum sulfidophilum
Biochemistry, 2001, 40, 10562 – 10569.
DOI: 10.1021/bi0100081

All Publications

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Chenge, J. T., Duyet, L. V., Swami, S., McLean, K. J., Kavanagh, M. E., Coyne, A. G., Rigby, S. E. J., Cheesman, M. R., Girvan, H. M., Levy, C. W., Rupp, B., von Kries, J. P., Abell, C., Leys, D., Munro, A. W.

(2017)

Structural Characterization and Ligand/Inhibitor Identification Provide Functional Insights into the Mycobacterium tuberculosis Cytochrome P450 CYP126A1,

in Journal of Biological Chemistry

292

(4)

pp. 1310-1329

Full Text UEA Repository

(Article)

(Published)


Pellicer Martinez, M., Bermejo Martinez, A., Crack, J., Holmes, J., Svistunenko, D., Johnston, A., Cheesman, M., Todd, J., Le Brun, N.

(2017)

Sensing iron availability via the fragile [4Fe-4S] cluster of the bacterial transcriptional repressor RirA,

in Chemical Science

8

pp. 8451-8463

Full Text UEA Repository

(Article)

(Published)


Girvan, H. M., Bradley, J. M., Cheesman, M. R., Kincaid, J. R., Liu, Y., Czarnecki, K., Fisher, K., Leys, D., Rigby, S. E. J., Munro, A. W.

(2016)

Analysis of Heme Iron Coordination in DGCR8: The Heme-Binding Component of the Microprocessor Complex,

in Biochemistry

55

(36)

pp. 5073-5083

Full Text UEA Repository

(Article)

(Published)


Lockwood, C., Burlat, B., Cheesman, M., Kern, M., Simon, J., Clarke, T., Richardson, D., Butt, J.

(2015)

Resolution of Key Roles for the Distal Pocket Histidine in Cytochrome c Nitrite Reductases,

in Journal of the American Chemical Society

137

(8)

pp. 3059–3068

Full Text UEA Repository

(Article)

(Published)


Van Wonderen, J. H., Oganesyan, V. S., Watmough, N. J., Richardson, D. J., Thomson, A. J., Cheesman, M. R.

(2013)

Unexpected weak magnetic exchange coupling between haem and non-haem iron in the catalytic site of nitric oxide reductase (NorBC) from Paracoccus denitrificans.,

in Biochemical Journal

451

(3)

pp. 389-394

Full Text UEA Repository

(Article)

(Published)


Doyle, R., Marritt, S., Gwyer, J., Lowe, T., Cheesman, M., Butt, J., Tikhonova, T., Popov, V.

(2013)

Contrasting catalytic profiles of multiheme nitrite reductases containing CxxCK heme-binding motifs,

in JBIC Journal of Biological Inorganic Chemistry

18

(6)

pp. 655-667

Full Text UEA Repository

(Article)

(Published)


Bradley, J., Marritt, S., Kihlken, M., Haynes, K., Hemmings, A., Berks, B., Cheesman, M., Butt, J.

(2012)

Redox and Chemical Activities of the Hemes in the Sulfur Oxidation Pathway Enzyme SoxAX,

in Journal of Biological Chemistry

287

(48)

pp. 40350-40359

Full Text UEA Repository

(Article)

(Published)


Marritt, S., Lowe, T., Bye, J., McMillan, D., Shi, L., Fredrickson, J., Zachara, J., Richardson, D., Cheesman, M., Jeuken, L., Butt, J.

(2012)

A functional description of CymA, an electron-transfer hub supporting anaerobic respiratory flexibility in Shewanella,

in Biochemical Journal

444

(3)

pp. 465-474

Full Text UEA Repository

(Article)

(Published)


Bradley, J., Butt, J., Cheesman, M.

(2011)

Electrochemical titrations and reaction time courses monitored in situ by magnetic circular dichroism spectroscopy,

in Analytical Biochemistry

419

(2)

pp. 110-116

Full Text UEA Repository

(Article)

(Published)


Bradley, J., Silkstone, G., Wilson, M., Cheesman, M., Butt, J.

(2011)

Probing a Complex of Cytochromecand Cardiolipin by Magnetic Circular Dichroism Spectroscopy: Implications for the Initial Events in Apoptosis,

in Journal of the American Chemical Society

133

(49)

pp. 19676-19679

Full Text UEA Repository

(Article)

(Published)


White, G., Singleton, C., Todd, J., Cheesman, M., Johnston, A., Le Brun, N.

(2011)

Heme binding to the second, lower-affinity site of the global iron regulator Irr from Rhizobium leguminosarum promotes oligomerization,

in FEBS Journal

278

(12)

pp. 2011-2021

Full Text UEA Repository

(Article)

(Published)


Driscoll, M. D., McLean, K. J., Cheesman, M. R., Jowitt, T. A., Howard, M., Carroll, P., Parish, T., Munro, A. W.

(2011)

Expression and characterization of Mycobacterium tuberculosis CYP144: Common themes and lessons learned in the M. tuberculosis P450 enzyme family,

in Biochimica et Biophysica Acta - Proteins and Proteomics

1814

(1)

pp. 76-87

Full Text UEA Repository

(Article)

(Published)


Kemp, G., Clarke, T., Marritt, S., Lockwood, C., Poock, S., Hemmings, A., Richardson, D., Cheesman, M., Butt, J.

(2010)

Kinetic and thermodynamic resolution of the interactions between sulfite and the pentahaem cytochrome NrfA fromEscherichia coli,

in Biochemical Journal

431

(1)

pp. 73-80

Full Text UEA Repository

(Article)

(Published)


Macmillan, F., Van Wonderen, J., Cheesman, M., Van Der Hocht, I., Angerer, H., Gennis, R., Michel, H.

(2010)

Using EPR up close and from afar: Elucidating mechanisms in haem copper oxidases,

in Biochimica et Biophysica Acta - Bioenergetics

1797

pp. 97

Full Text UEA Repository

(Article)

(Published)


Singleton, C., White, G., Todd, J., Marritt, S., Cheesman, M., Johnston, A., Le Brun, N.

(2010)

Heme-responsive DNA binding by the global iron regulator Irr from Rhizobium leguminosarum,

in The Journal of Biological Chemistry

285

(21)

pp. 16023-16031

Full Text UEA Repository

(Article)

(Published)


Girvan, H. M., Levy, C. W., Williams, P., Fisher, K., Cheesman, M. R., Rigby, S. E. J., Leys, D., Munro, A. W.

(2010)

Glutamate-haem ester bond formation is disfavoured in flavocytochrome P450 BM3: characterization of glutamate substitution mutants at the haem site of P450 BM3,

in Biochemical Journal

427

pp. 455-466

Full Text UEA Repository

(Article)

(Published)


Jervis, A., Crack, J., White, G., Artymiuk, P., Cheesman, M., Thomson, A., Le Brun, N., Green, J.

(2009)

The O2 sensitivity of the transcription factor FNR is controlled by Ser24 modulating the kinetics of [4Fe-4S] to [2Fe-2S] conversion,

in Proceedings of the National Academy of Sciences of the United States of America

106

(12)

pp. 4659-4664

Full Text UEA Repository

(Article)

(Published)


Bew, S., Cheesman, M., Sharma, S.

(2009)

Ferrocenium Salts Mediate para-tert-Butylcalixarene Synthesis.,

in ChemInform

40

(13)

Full Text UEA Repository

(Article)

(Published)


Girvan, H. M., Toogood, H. S., Littleford, R. E., Seward, H. E., Smith, W. E., Ekanem, I. S., Leys, D., Cheesman, M. R., Munro, A. W.

(2009)

Novel haem co-ordination variants of flavocytochrome P450 BM3,

in Biochemical Journal

417

pp. 65-76

Full Text UEA Repository

(Article)

(Published)


Kemp, G., Marritt, S., Xiaoe, L., Durrant, J., Cheesman, M., Butt, J.

(2009)

Opportunities for mesoporous nanocrystalline SnO2 electrodes in kinetic and catalytic analyses of redox proteins,

in Biochemical Society Transactions

37

(2)

pp. 368-372

Full Text UEA Repository

(Article)

(Published)


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Key Research Interests

Research Funding

EPSRC
Molecular nanomagnets: Synthetic and spectroscopic exploration of the limits of information storage
Thomson, AJ and Cheesman, MR
01-Oct-03 - 30-Jun-09
£327,877

BBSRC
The molecular enzymology of nitric oxide reductase: cellular saviour and global bandit
Watmough, NJ, Butt, JN, Cheesman, MR and Richardson, DJ
01-Sep-05 - 31-Aug-08
£221,432

BBSRC
Mottle-MCD Spectroscopy with in situ electrochemical control
Butt, JN, Cheesman, MR and Durrant, J (Imperial)
01-Aug-05 - 31-Jul-08
£146,297

BBSRC
Signal perception by the global transcription factor FNR
Thomson, AJ, Cheesman, MR and LeBrun, NE
15-Nov-04 - 14-Nov-07
£251,717

BBSRC
Relaxation of electron spins by anisotropic paramagnetic metal centres; a novel probe of protein structure and dynamics
Thomson, AJ and Cheesman, MR
01-Aug-04 - 30-Sep-07
£227,361

BBSRC
The cytochrome c nitrate reductase: an integrated study of nitrite and nitric oxide reduction by a deca-heme enzyme complex
Richardson, DJ, Butt, JN, Cheesman, MR, Hemmings, AM and Cole, J (Birmingham)
20-Oct-03 - 19-Jul-07
£354,500

Wellcome Trust
JIF: Biophysical Chemistry at UEA
Thomson, AJ, Cheesman, MR, Le Brun, NE, Kleanthous, CK (York), Richardson, DJ, Watmough, NJ, Moore, GR, Butt, JN, Hemmings, AM and Hayward, S
01-Jun-01 - 30-Jun-06
£3,457,321

BBSRC
Mapping of membrane proteins by multi-frequency time domain EPR
Thomson, AJ and Cheesman, MR
01-Oct-02 - 16-Jun-06
£181,016

BBSRC
Structure-function studies of a novel haemoprotein involved in bacterial oxidation of thiosulfate & sulfide
Hemmings, AM, Butt, JN, Cheesman, MR and Berks, B (Oxford)
01-Jan-02 - 30-Apr-05
£42,300

BBSRC
Structure and function of nitric oxide reducatase: An ancient oxidase
Thomson, A J, Cheesman, M R, Watmough, N, Richardson, D J & Spiro S
15-Nov-98 - 25-Nov-02
£207,624

Research Group Membership

Postdoc

  • Sophie Marritt
  • Jessica van Wonderen
  • Gaye White
  • Justin Bradley
  • Sarah Field

PhD

  • Susan Bennett
  • John Holmes
  • Kate Haynes
  • Gemma Kemp
  • Louise Ottignon