Biography

School Position:

Emeritus Professor of Biology and Chemistry
 
Andrew Thomson’s major research interests throughout his career at UEA as founder Director of the Centre for Metalloprotein Spectroscopy and Biology, (CMSB) have been in the application of optical and magnetic methods to analyse the structures and functions of transition metal centres in proteins. Magnetic circular dichroism (MCD) and electron paramagnetic resonance (EPR) spectroscopy as well as novel optical-microwave double resonance techniques have been developed. The systems studied include enzymes of the bacterial denitrification pathway that contain hemoproteins, iron-sulfur and multi-copper clusters as well terminal heme-copper oxidases. proteins of iron uptake and storage pathways and proteins that utilise iron sulphur clusters to regulate gene expression. In 2005 Andrew Thomson established the Henry Wellcome Unit for Biological EPR Spectroscopy (Director Dr Fraser MacMillan) at UEA utilizing multi-frequency (9 and 95 GHz) and time domain EPR to map membrane metalloproteins using site directed spin labelling.

Early in his career after completing his PhD degree with RJP Williams in Oxford Andrew Thomson worked in the laboratory of B Rosenberg, between 1965-67, on the effect of platinum salts on bacterial growth processes. He was the first to show that the cis isomer of dichlorodiammineplatinum(II) (cisPlatin) was the effective biological agent in inhibiting cell divsion in E.coli. This led ultimatley to its clinical use as a highly effective chemotherapeutic agent against several cancers particularly testicular cancer.

Awards

  • 1993 Elected Fellow Royal Society of London
  • 1991 Royal Society of Chemistry Silver Medal for Analytical Spectroscopy.
  • 1998 Royal Society of Chemistry Hugo Müller Medal and Lectureship
  • 2002 Royal Society of Chemistry Interdisciplinary Award
  • 2004 Royal Society of Chemistry Chatt Lectureship and medal
  • 2008 Awarded OBE for services to Higher Education

Selected Publications

Analysis of nitroxide spin label motions in a protein-protein complex using multiple frequency EPR spectroscopy.
Gaye F White, Louise Ottignon, Toni Georgiou, Colin Kleanthous, Geoff R Moore Andrew J Thomson, Vasily S Oganesyan.
J. Mag. Res. 2007 185, 191-203.
DOI: 10.1016/j.jmr.2006.12.009

Superoxide mediated-amplification of the oxygen-induced switch from [4Fe-4S] to [2Fe-2S] clusters in the transcriptional regulator FNR.
Jason C. Crack, Jeffrey Green, Myles R. Cheesman, Nick E. Le Brun and Andrew J. Thomson.
Proc. Natl. Acad. Sci. USA 2007 104, 2092-2097.
DOI:10.1073/pnas.0609514104

Magnetic circular dichroism anisotropy of the CuA centre of nitrous oxide reductase from coherent Raman detected electron spin resonance spectroscopy.
Stephen J Bingham, Tim Rasmussen, Jaqui Farrar, Daniel Wolverson, Andrew J. Thomson.
Molecular Physics 2007 105, 2169 - 2176.
DOI: 10.1080/00268970701732985

Influence of the Environment on the [4Fe-4S]2+ to [2Fe-2S]2+ Cluster Switch in the Transcriptional Regulator FNR.
Jason C. Crack, Jeffrey Green, Myles R. Cheesman, Nick E. Le Brun, and Andrew J. Thomson
J. Am. Chem. Soc. 2008 130 1749 – 1758.
DOI: 10.1021/ja077455+

Magnetic Circular Dichroism Spectroscopy of Antiferromagnetically Coupled Hetero-Metallic Rings [H2NR2][Cr7MF8(O2CCMe3)16].
Justin M. Bradley, Andrew J. Thomson, Eric J. McInnes, Richard E.P. Winpenny, Grigore Timco.
Dalton Trans. 2008 3311-3319
DOI: 10.1039/b803269f

Mitigating release of the potent greenhouse gas N2O from the nitrogen cycle – could enzymic regulation hold the key?
David Richardson, Heather Felgate, Nick Watmough, Andrew Thomson and Elizabeth Baggs.
Trends Biotechnol. 2009 27, 385-442

Optimizing the sensitivity to O2 of the transcription factor FNR: Ser24 modulates the kinetics of the [4Fe-4S] to [2Fe-2S] conversion.
AJ Jervis, JC Crack, G White, PJ Artymiuk, MR Cheesman, AJ Thomson, NE Le Brun, J Green.
Proc. Natl. Acad. Sci. USA. 2009 106, 4659-64

Wellcome Trust Witnesses to Twentieth Century Medicine Volume 30. “The Discovery, Use and Impact of Platinum Salts as Chemotherapy Agent for Cancer”.
Ed. DA Christie, EM Tansey.
Wellcome Trust Centre for the History of Medicine. UCL, 2007. ISBN 978 085484 112 7.
Pp 6-15 Contribution from Andrew J Thomson

Subunit Organization in the TatA Complex of the Twin Arginine Protein Translocase. A site-directed EPR spin labeling study.
Gaye F. White, Sonya M. Schermann, Justin Bradley, Andrew Roberts, Nicholas P. Greene, Ben C. Berks, and Andrew J. Thomson.
J. Biol Chem 2010 285 (no. 4) 2294 - 2301
DOI: 10.1074/jbc.M109.065458

All Publications

<- Page 1 of 4 ->

Dodd, E., Crack, J., Thomson, A., Le Brun, N.

(2017)

Reactivity of iron-sulfur clusters with nitric oxide,

in Iron-Sulfur Clusters in Chemistry and Biology : Volume 1: Characterization, Properties and Applications.

De Gruyter

ISBN 978-3-11-048043-6

Full Text

(Chapter (peer-reviewed))

(Published)


Crack, J. C., Thomson, A. J., Le Brun, N. E.

(2017)

Mass spectrometric identification of intermediates in the O2-driven [4Fe-4S] to [2Fe-2S] cluster conversion in FNR,

in Proceedings of the National Academy of Sciences of the United States of America (PNAS)

114

(6)

pp. E3215–E3223

Full Text UEA Repository

(Article)

(Published)


Oganesyan, V. S., Chami, F., White, G. F., Thomson, A. J.

(2017)

A combined EPR and MD simulation study of a nitroxyl spin label with restricted internal mobility sensitive to protein dynamics,

in Journal of Magnetic Resonance

274

pp. 24–35

Full Text UEA Repository

(Article)

(Published)


Serrano, P. N., Wang, H., Crack, J. C., Prior, C., Hutchings, M. I., Thomson, A. J., Kamali, S., Yoda, Y., Zhao, J., Hu, M. Y., Alp, E. E., Oganesyan, V. S., Le Brun, N. E., Cramer, S. P.

(2016)

Nitrosylation of Nitric-Oxide-Sensing Regulatory Proteins Containing [4Fe-4S] Clusters Gives Rise to Multiple Iron-Nitrosyl Complexes,

in Angewandte Chemie International Edition

55

(47)

pp. 14575–14579

Full Text UEA Repository

(Article)

(Published)


Crack, J., Svistunenko, D. A., Munnoch, J., Thomson, A., Hutchings, M., Le Brun, N.

(2016)

Differentiated, promoter-specific response of [4Fe-4S] NsrR DNA-binding to reaction with nitric oxide,

in Journal of Biological Chemistry

291

pp. 8663-8672

Full Text UEA Repository

(Article)

(Published)


Crack, J., Hutchings, M., Thomson, A., Le Brun, N.

(2016)

Biochemical properties of Paracoccus denitrificans FnrP: Reactions with molecular oxygen and nitric oxide,

in JBIC Journal of Biological Inorganic Chemistry

21

(1)

pp. 71-82

Full Text UEA Repository

(Article)

(Published)


Ibrahim, S. A., Crack, J. C., Rolfe, M. D., Borrero-de Acuňa, J. M., Thomson, A. J., Le Brun, N. E., Schobert, M., Stapleton, M. R., Green, J.

(2015)

Three Pseudomonas putida FNR Family Proteins with Different Sensitivities to O2,

in The Journal of Biological Chemistry

290

pp. 16812-16823

Full Text UEA Repository

(Article)

(Published)


Crack, J., Munnoch, J. T., Dodd, E., Knowles, F., Al-Bassam, M., Kamali, S., Holland, A., Cramer, S., Hamilton, C., Johnson, M., Thomson, A., Hutchings, M., Le Brun, N.

(2015)

NsrR from Streptomyces coelicolor is a nitric oxide-sensing [4Fe-4S] cluster protein with a specialized regulatory function,

in The Journal of Biological Chemistry

290

pp. 12689-12704

Full Text UEA Repository

(Article)

(Published)


Crack, J. C., Stapleton, M. R., Green, J., Thomson, A. J., Le Brun, N. E.

(2014)

Influence of association state and DNA binding on the O2-reactivity of [4Fe-4S] fumarate and nitrate reduction (FNR) regulator,

in Biochemical Journal

463

(1)

pp. 83-92

Full Text UEA Repository

(Article)

(Published)


Crack, J. C., Green, J., Thomson, A. J., Le Brun, N. E.

(2014)

Iron-sulfur clusters as biological sensors: the chemistry of reactions with molecular oxygen and nitric oxide,

in Accounts of Chemical Research

47

(10)

pp. 3196-205

Full Text UEA Repository

(Article)

(Published)


Van Wonderen, J., McMahon, R., OMara, M., McDevitt, C., Thomson, A., Kerr, I., MacMillan, F., Callaghan, R.

(2014)

The central cavity of ABCB1 undergoes alternating access during ATP hydrolysis,

in FEBS Journal

281

(9)

pp. 2190-2201

Full Text UEA Repository

(Article)

(Published)


Crack, J. C., Green, J., Thomson, A. J., Le Brun, N. E.

(2014)

Techniques for the production, isolation, and analysis of iron-sulfur proteins,

in Methods in Molecular Biology

1122

pp. 33-48

Full Text UEA Repository

(Article)

(Published)


Van Wonderen, J. H., Oganesyan, V. S., Watmough, N. J., Richardson, D. J., Thomson, A. J., Cheesman, M. R.

(2013)

Unexpected weak magnetic exchange coupling between haem and non-haem iron in the catalytic site of nitric oxide reductase (NorBC) from Paracoccus denitrificans.,

in Biochemical Journal

451

(3)

pp. 389-394

Full Text UEA Repository

(Article)

(Published)


Crack, J. C., Stapleton, M. R., Green, J., Thomson, A. J., Le Brun, N. E.

(2013)

Mechanism of [4Fe-4S](Cys)4 cluster nitrosylation is conserved among NO-responsive regulators,

in The Journal of Biological Chemistry

288

(16)

pp. 11492-502

Full Text UEA Repository

(Article)

(Published)


Thomson, A. J., Giannopoulos, G., Pretty, J., Baggs, E. M., Richardson, D. J.

(2012)

Biological sources and sinks of nitrous oxide and strategies to mitigate emissions,

in Philosophical Transactions of the Royal Society B: Biological Sciences

367

(1593)

pp. 1157-1168

Full Text UEA Repository

(Article)

(Published)


Crack, J., Green, J., Hutchings, M., Thomson, A., Le Brun, N.

(2012)

Bacterial iron-sulfur regulatory proteins as biological sensor-switches,

in Antioxidants & Redox Signaling

17

pp. 1215-1231

Full Text UEA Repository

(Article)

(Published)


Crack, J., Green, J., Thomson, A., Le Brun, N.

(2012)

Iron–sulfur sensor-regulators,

in Current Opinion in Chemical Biology

16

pp. 35-44

Full Text UEA Repository

(Article)

(Published)


Zhang, B., Crack, J., Subramanian, S., Green, J., Thomson, A., Le Brun, N., Johnson, M.

(2012)

Reversible cycling between cysteine persulfide-ligated [2Fe-2S] and cysteine-ligated [4Fe-4S] clusters in the FNR regulatory protein,

in Proceedings of the National Academy of Sciences

109

(39)

pp. 15734-15739

Full Text UEA Repository

(Article)

(Published)


Zhang, B., Crack, J., Subramanian, S., Green, J., Thomson, A., Le Brun, N., Johnson, M.

(2012)

Reversible cycling between partially cysteine persulfide-ligated [2Fe-2S] clusters and cysteine ligated [4Fe-4S] clusters in FNR,

in Proceedings of the National Academy of Sciences of the United States of America

109

(39)

pp. 15734-15739

UEA Repository

(Article)

(Published)


Crack, J., Smith, L., Stapleton, M., Peck, J., Watmough, N., Buttner, M., Buxton, R., Green, J., Oganesyan, V., Thomson, A., Le Brun, N.

(2011)

Mechanistic Insight into the Nitrosylation of the [4Fe−4S] Cluster of WhiB-like Proteins,

in Journal of the American Chemical Society

133

(4)

pp. 1112-1121

Full Text UEA Repository

(Article)

(Published)


<- Page 1 of 4 ->

Key Research Interests

Research Funding

EPSRC
Molecular nanomagnets: Synthetic and spectroscopic exploration of the limits of information storage
Thomson, AJ and Cheesman, MR
01-Oct-03 - 30-Jun-09
£327,877

BBSRC
The Wbl proteins - a novel family of [4Fe-4S] cluster-containing transcription factors
Thomson, J
01-Apr-06 - 31-Mar-09
£204,701

BBSRC
Signal perception by the global transcription factor FNR
Thomson, AJ, Cheesman, MR and LeBrun, NE
15-Nov-04 - 14-Nov-07
£251,717

BBSRC
Relaxation of electron spins by anisotropic paramagnetic metal centres; a novel probe of protein structure and dynamics
Thomson, AJ and Cheesman, MR
01-Aug-04 - 30-Sep-07
£227,361

Wellcome Trust
JIF: Biophysical Chemistry at UEA
Thomson, AJ, Cheesman, MR, Le Brun, NE, Kleanthous, CK (York), Richardson, DJ, Watmough, NJ, Moore, GR, Butt, JN, Hemmings, AM and Hayward, S
01-Jun-01 - 30-Jun-06
£3,457,321

BBSRC
Mapping of membrane proteins by multi-frequency time domain EPR
Thomson, AJ and Cheesman, MR
01-Oct-02 - 16-Jun-06
£181,016

HEFCE
SRIF 2 - Chemical Science Research Infrastructure
Thomson, AJ
15-Dec-05 - 31-Mar-06
£100,000

Royal Society of Chemistry
Protein specific spectroscopic labels for screening of drug-cell interactions by time domain multi-frequency EPR
Thomson, AJ
01-Oct-02 - 31-Dec-05
£44,610

BBSRC
The bioenergetics and biosynthesis of the bacterial nitric oxide reductase
Richardson, DJ, Thomson, AJ, Spiro, S and Watmough, NJ
01-Oct-00 - 30-Apr-04
£197,816

HEFCE
JREI: A bacterial growth downstream processing facility at UEA
Richardson, DJ, Thomson, AJ + 6 others
26-Mar-01 - 01-Dec-03
£199,634

BBSRC
Metalloprotein spectroscopy & structural biology of microbial and plant proteins
Thomson, A J, Moore, G R, Richardson, D J & Kleanthous, K C
01-Oct-99 - 30-Sep-03
£912,408

Institute of Food Research
Contribution to Chemistry Research
Thomson, AJ
01-Jul-01 - 30-Jun-03
£120,000

Wellcome Trust
Structural spectroscopic and electrochemical characterisation of tetra-heme and deca-heme cytochromes involved in bacterial Fe(III) respiration
Thomson, AJ, Richardson, D J & Hemmings, AM
01-Jan-00 - 01-May-03
£277,510

BBSRC
Development of two photon fluorescence bioimaging
Meech, S R, Andrews, D L, Thomson, A J & Shaw, P J (John Innes Centre, Norwich)
13-Oct-99 - 13-Mar-03
£189,564

BBSRC
Monitoring Environmental Oxygen: FNR, A sensor of Oxygen and Oxidative Stress
Thomson, A J and Green, J G (Sheffield)
01-Jan-00 - 31-Dec-02
£59,301

BBSRC
Structure and function of nitric oxide reductase: An ancient oxidase
Thomson, A J, Cheesman, M R, Watmough, N, Richardson, D J & Spiro S
15-Nov-98 - 25-Nov-02
£207,624

BBSRC
Studentship
Thomson, AJ
01-Oct-99 - 30-Sep-02

BBSRC
Nitrous oxide reductase: Comparative and functional studies of novel copper-thiolate centres
Thomson, A J and Richardson, DJ
01-Jul-98 - 31-May-02
£163,148

EPSRC
Magneto-optical properties & synthesis of molecular magnets of transition metal clusters
Thomson, A J and Powell, A K
01-Jan-99 - 31-Dec-01
£157,645

Royal Society of Chemistry
Development of a sensitive and selective direct electrochemical sensor for DNA with potential application in parallel genomics
Thomson, A J and Butt, JN
01-Oct-98 - 30-Sep-01
£32,000

EPSRC
Supramolecular arrays M(111) oxyhydroxide nanodisks: Synthesis, structures, magnetism and film formation
Thomson, A J and Heath, S L (Sheffield)
01-Oct-97 - 31-Jan-01
£133,575