Biography

PhD studentship opportunity: We currently have one BBSRC-funded PhD studentship available in our group. Please contact Amit Sachdeva (a.sachdeva@uea.ac.uk) directly if you are interested in applying for this position.  Read more - Modulating properties of proteins with non-natural amino acids (SACHDEVA_U17DTP)

Amit Sachdeva is a lecturer in Bio-organic Chemistry at UEA and his research interests are in the area of Chemical Biology and Synthetic Biology. Amit completed his PhD from the University of Illinois at Urbana-Champaign, where he was involved in identification of DNA-based enzymes with potential for biomedical and diagnostic applications. He then moved to Cambridge for postdoctoral work at the MRC Laboratory of Molecular Biology. Here, he was involved in developing methods to enable synthesis of proteins containing multiple non-natural amino acids in live cells.

At the University of East Anglia, Amit’s research group is developing methods to genetically encode new unnatural amino acids that would provide access to several medically important proteins. His research group is also focusing its efforts on modulating properties of proteins to develop novel therapeutics and medical diagnosis tools.

Selected publications

D.T. Rogerson, A. Sachdeva, K. Wang, T. Haq, A. Kazlauskaite, S.M. Hancock, N. Huguenin-Dezot, M.M.K. Muqit, A.M. Fry, R. Bayliss and and J.W. Chin.
Efficient genetic encoding of phosphoserine and its non-hydrolyzable analog.
Nature Chemical Biology, online, 2015, 11, 496-503.

A. Sachdeva, K. Wang, T.S. Elliott and and J.W. Chin.
Concerted, rapid, quantitative and site-specific dual labeling of protein.
Journal of the American Chemical Society (JACS), 2014, 136, 7785-7788.

K. Wang*, A. Sachdeva*, D.J. Cox, N.W. Wilf, K. Lang, S. Wallace R.A. Mehl and J.W. Chin.
Optimized orthogonal translation of unnatural amino acids enables spontaneous protein double labelling and FRET.
Nature Chemistry, 2014, 6, 393-403. (*=Co-first authors)

T.S. Elliott, F.M. Townsley, A. Bianco, R.J. Ernst, A. Sachdeva, S.J. Elsässer, L. Davis, K. Lang, R. Pisa, S. Greiss, Kathryn S. Lilley and J.W. Chin.
Proteome Labeling and Protein Identification in Specific Tissues and at Specific Development Stages in an Animal.
Nature Biotechnology, 2014, 32, 465-472.

S.M. Walsh, A. Sachdeva and S.K. Silverman.
DNA catalysts with tyrosine kinase activity.
Journal of the American Chemical Society (JACS), 2013, 135, 14928-14931.

All Publications

Rogerson, D., Sachdeva, A., Wang, K., Haq, T., Kazlauskaite, A., Hancock, S., Hugenin-Dezot, N., Muqit, M., Fry, A., Bayliss, R., Chin, J.

(2015)

Efficient genetic encoding of phosphoserine and its non-hydrolyzable analog,

in Nature Chemical Biology

11

(7)

pp. 496–503

Full Text UEA Repository

(Article)

(Published)


Brandsen, B. M., Velez, T. E., Sachdeva, A., Ibrahim, N. A., Silverman, S. K.

(2014)

DNA-Catalyzed Lysine Side Chain Modification,

in Angewandte Chemie International Edition

53

(34)

pp. 9045-9050

Full Text UEA Repository

(Article)

(Published)


Sachdeva, A., Wang, K., Elliott, T., Chin, J. W.

(2014)

Concerted, Rapid, Quantitative, and Site-Specific Dual Labeling of Proteins,

in Journal of the American Chemical Society

136

(22)

pp. 7785-7788

Full Text UEA Repository

(Article)

(Published)


Elliott, T. S., Townsley, F. M., Bianco, A., Ernst, R. J., Sachdeva, A., Elsässer, S. J., Davis, L., Lang, K., Pisa, R., Greiss, S., Lilley, K. S., Chin, J. W.

(2014)

Proteome labeling and protein identification in specific tissues and at specific developmental stages in an animal,

in Nature Biotechnology

32

(5)

pp. 465-472

Full Text UEA Repository

(Article)

(Published)


Wang, K., Sachdeva, A., Cox, D. J., Wilf, N. W., Lang, K., Wallace, S., Mehl, R. A., Chin, J. W.

(2014)

Optimized orthogonal translation of unnatural amino acids enables spontaneous protein double-labelling and FRET,

in Nature Chemistry

6

(5)

pp. 393-403

Full Text UEA Repository

(Article)

(Published)


Walsh, S. M., Sachdeva, A., Silverman, S. K.

(2013)

DNA Catalysts with Tyrosine Kinase Activity,

in Journal of the American Chemical Society

135

(40)

pp. 14928-14931

Full Text UEA Repository

(Article)

(Published)


Sachdeva, A., Chandra, M., Chandrasekar, J., Silverman, S. K.

(2012)

Covalent Tagging of Phosphorylated Peptides by Phosphate-Specific Deoxyribozymes,

in ChemBioChem

13

(5)

pp. 654-657

Full Text UEA Repository

(Article)

(Published)


Sachdeva, A., Silverman, S. K.

(2012)

DNA-catalyzed reactivity of a phosphoramidate functional group and formation of an unusual pyrophosphoramidate linkage,

in Organic & Biomolecular Chemistry

10

(1)

pp. 122-125

Full Text UEA Repository

(Article)

(Published)


Sachdeva, A., Silverman, S. K.

(2010)

DNA-catalyzed serine side chain reactivity and selectivity,

in Chemical Communications

46

(13)

pp. 2215

Full Text UEA Repository

(Article)

(Published)


Chandra, M., Sachdeva, A., Silverman, S. K.

(2009)

DNA-catalyzed sequence-specific hydrolysis of DNA,

in Nature Chemical Biology

5

(10)

pp. 718-720

Full Text UEA Repository

(Article)

(Published)


Sudarshan, K., Rath, S., Patri, M., Sachdeva, A., Pujari, P.

(2007)

Positron annihilation spectroscopic studies of fluorinated ethylene propylene copolymer-g-polystyrene,

in Polymer

48

(21)

pp. 6434-6438

Full Text UEA Repository

(Article)

(Published)


Kaiser, M., Nambissan, P., Banerjee, M., Sachdeva, A., Pujari, P.

(2007)

Positron lifetime studies and coincidence Doppler broadening spectroscopy of Al-6Mg-xSc (x= 0 to 0.6 wt.%) alloy,

in Journal of Materials Science

42

Full Text UEA Repository

(Article)

(Published)


Dutta, D., Sachdeva, A., Pujari, P.

(2006)

Positron annihilation studies on the phase transition of benzene and reactivity of nitrobenzene in confined framework of ZSM-5 zeolite,

in Chemical Physics Letters

432

(1-3)

pp. 116-121

Full Text UEA Repository

(Article)

(Published)


Sachdeva, A., Sodaye, S., Pandey, A. K., Goswami, A.

(2006)

Formation of Silver Nanoparticles in Poly(perfluorosulfonic) Acid Membrane,

in Analytical Chemistry

78

(20)

pp. 7169-7174

Full Text UEA Repository

(Article)

(Published)


Sachdeva, A., Chavan, S., Goswami, A., Tyagi, A., Pujari, P.

(2005)

Positron annihilation spectroscopic studies on Nd-doped ceria,

in Journal of Solid State Chemistry

178

(6)

pp. 2062-2066

Full Text UEA Repository

(Article)

(Published)


Sachdeva, A., Sudarshan, K., Pujari, P. K., Goswami, A., Sreejith, K., George, V. C., Pillai, C. G. S., Dua, A. K.

(2004)

Identification of nitrogen decorated vacancies in CVD diamond films using positron annihilation coincidence Doppler broadening spectroscopy,

in Diamond and Related Materials

13

(9)

pp. 1719-1724

Full Text UEA Repository

(Article)

(Published)


Dutt, G. B., Sachdeva, A.

(2003)

Temperature-dependent rotational relaxation in a viscous alkane: Interplay of shape factor and boundary condition on molecular rotation,

in The Journal of Chemical Physics

118

(18)

article no. 8307

Full Text UEA Repository

(Article)

(Published)


Key Research Interests

Nature elegantly orchestrates complex chemical and physical transformations to accomplish functions as diverse as transmitting information across generations to harvesting energy from sunlight. These natural processes are achieved by the limited chemical functionality available to biology. Expanding the repertoire of chemical functional groups available to biology can lead to development of new biological systems with potential applications in biotechnology, biofuels and medicine. The focus of our current research work is in developing methods to enable genetic incorporation of structurally diverse non-natural amino acids into proteins and also, to employ these non-natural amino acids to create novel proteins that can be used in medicine.

We currently have one fully funded PhD position available. Please follow the link to apply for this position. Please email me directly at a.sachdeva@uea.ac.uk for alternative sources of funding or to discuss potential projects.

If you are interested in a postdoctoral position in our laboratory, please email me directly. We are very interested in potential postdocs with a strong background in cell biology, synthetic biology or chemical biology. Applicants are encouraged to look for funding sources, including Wellcome Trust Postdoctoral Fellowship, Marie Curie Fellowships, Newton Fellowship.