Career History

  • Lecturer, University of East Anglia (2011-present) 
  • RCUK research Fellow, University of East Anglia (2006-2011)
  • Senior Research Associate, University of East Anglia (2003-2006)
  • Research Fellow, University of Michigan (2001-2003)
  • Ph.D. John Innes Centre, University of East Anglia (1998-2001)
  • M.Sc. Biomolecular Technology, University of Leicester (1997)
  • B.Sc. Biochemistry, University College London, University of London (1992-1995)


ResearcherID  

http://www.researcherid.com/rid/D-1837-2009  

Highlighted Publications

See All

Edwards, M., White, G., Norman, M., Tome-Fernandez, A., Ainsworth, E., Shi, L., Fredrickson, J., Zachara, J., Butt, J., Richardson, D., Clarke, T.

(2015)

Redox Linked Flavin Sites in Extracellular Decaheme Proteins Involved in Microbe-Mineral Electron Transfer,

in Scientific Reports

5.

Full Text UEA Repository

(Article)

(Published)


Clarke, T., Edwards, M., Gates, A., Hall, A., White, G., Bradley, J., Reardon, C., Shi, L., Beliaev, A., Marshall, M., Wang, Z., Watmough, N., Fredrickson, J., Zachara, J., Butt, J., Richardson, D.

(2011)

Structure of a bacterial cell surface decaheme electron conduit,

in Proceedings of the National Academy of Sciences

108.

pp. 9384-9389

Full Text UEA Repository

(Article)

(Published)


All Publications

See Highlights
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Davies, J. A., Clarke, T. A., Butt, J. N.

(2017)

Making Connections: An Amphiphilic Ferrocene Stimulates Bacterial Electricity Production,

in Chem

2

(2)

pp. 164-167

Full Text

(Comment/debate)

(Published)


Hartop, K., Sullivan, M., Giannopoulos, G., Gates, A., Bond, P., Yuan, Z., Clarke, T., Rowley, G., Richardson, D.

(2017)

The metabolic impact of extracellular nitrite on aerobic metabolism of Paracoccus denitrificans,

in Water Research

113

pp. 207–214

Full Text UEA Repository

(Article)

(Published)


White, G. F., Edwards, M. J., Gomez-Perez, L., Richardson, D. J., Butt, J. N., Clarke, T. A.

(2016)

Mechanisms of Bacterial Extracellular Electron Exchange.,

in Advances in Microbial Physiology : Advances in Bacterial Electron Transport Systems and Their Regulation.

Elsevier

pp. 87-138

ISBN 978-0-12-804822-1

Full Text UEA Repository

(Chapter)

(Published)


Norman, M., White, G., Edwards, M., Clarke, T.

(2016)

Flavocytochrome at the microbe-mineral interface of Shewanella oneidensis under mineral respiring conditions,

in New Biotechnology

33

pp. S60-S61

Full Text

(Abstract)

(Published)


Davies, J., Clarke, T., Butt, J., Richardson, D.

(2016)

Carbon fixation via the formate dehydrogenases of Shewanella,

in New Biotechnology

33

pp. S110

Full Text

(Abstract)

(Published)


Ainsworth, E. V., Lockwood, C. W. J., White, G. F., Hwang, E. T., Sakai, T., Gross, M. A., Richardson, D. J., Clarke, T. A., Jeuken, L. J. C., Reisner, E., Butt, J. N.

(2016)

Photoreduction of Shewanella oneidensis Extracellular Cytochromes by Organic Chromophores and Dye-Sensitized TiO2,

in ChemBioChem

17

(24)

pp. 2324–2333

Full Text UEA Repository

(Article)

(Published)


Field, S., Ryden, P., Wilson, D. C., James, S. A., Roberts, I., Richardson, D., Waldron, K., Clarke, T.

(2015)

Identification of furfural resistant strains of Saccharomyces cerevisiae and Saccharomyces paradoxus from a collection of environmental and industrial isolates,

in Biotechnology for Biofuels

8

article no. 33

Full Text UEA Repository

(Article)

(Published)


Lockwood, C., Burlat, B., Cheesman, M., Kern, M., Simon, J., Clarke, T., Richardson, D., Butt, J.

(2015)

Resolution of Key Roles for the Distal Pocket Histidine in Cytochrome c Nitrite Reductases,

in Journal of the American Chemical Society

137

(8)

pp. 3059–3068

Full Text UEA Repository

(Article)

(Published)


Beckwith, C., Edwards, M., Lawes, M., Shi, L., Butt, J., Richardson, D., Clarke, T.

(2015)

Characterisation of MtoD from Sideroxydans lithotrophicus: a cytochrome c electron shuttle used in lithoautotrophic growth,

in Frontiers in Microbiology

6

article no. 332

Full Text UEA Repository

(Article)

(Published)


Wang, Z., Shi, Z., Shi, L., White, G., Richardson, D., Clarke, T., Fredrickson, J., Zachara, J.

(2015)

Effects of soluble flavin on heterogeneous electron transfer between surface exposed bacterial cytochromes and iron oxides,

in Geochimica et Cosmochimica Acta

163

pp. 299–310

Full Text UEA Repository

(Article)

(Published)


Edwards, M., White, G., Norman, M., Tome-Fernandez, A., Ainsworth, E., Shi, L., Fredrickson, J., Zachara, J., Butt, J., Richardson, D., Clarke, T.

(2015)

Redox Linked Flavin Sites in Extracellular Decaheme Proteins Involved in Microbe-Mineral Electron Transfer,

in Scientific Reports

5

article no. 11677

Full Text UEA Repository

(Article)

(Published)


Edwards, M. J., Baiden, N., Johs, A., Tomanicek, S. J., Liang, L., Shi, L., Fredrickson, J. K., Zachara, J. M., Gates, A. J., Butt, J. N., Richardson, D. J., Clarke, T. A.

(2014)

The X-ray crystal structure of Shewanella oneidensis OmcA reveals new insight at the microbe-mineral interface,

in FEBS Letters

588

(10)

pp. 1886-1890

Full Text UEA Repository

(Article)

(Published)


Liu, Y., Wang, Z., Liu, J., Levar, C., Edwards, M., Babauta, J., Kennedy, D., Shi, Z., Bayenal, H., Bond, D. R., Clarke, T., Butt, J., Richardson, D., Rosso, K., Zachara, J., Fredrickson, J., Shi, L.

(2014)

A trans-outer membrane porin-cytochrome protein complex for extracellular electron transfer by Geobacter sulfurreducens PCA,

in Environmental Microbiology Reports

6

(6)

pp. 776-785

Full Text UEA Repository

(Article)

(Published)


White, G., Shi, Z., Shi, L., Wang, Z., Dohnalkova, A. C., Marshall, M. J., Fredrickson, J. K., Zachara, J. M., Butt, J., Richardson, D., Clarke, T.

(2013)

Rapid electron exchange between surface-exposed bacterial cytochromes and Fe(III) minerals.,

in Proceedings of the National Academy of Sciences of the United States of America (PNAS)

110

(16)

pp. 6346-6351

Full Text UEA Repository

(Article)

(Published)


Richardson, D., Butt, J., Clarke, T.

(2013)

Controlling electron transfer at the microbe-mineral interface,

in Proceedings of the National Academy of Sciences of the United States of America (PNAS)

110

(19)

pp. 7537-7538

Full Text UEA Repository

(Article)

(Published)


Doyle, R. A., Richardson, D. J., Clarke, T. A., Butt, J. N.

(2013)

Freely diffusing versus adsorbed protein: Which better mimics the cellular state of a redox protein?,

in Electrochimica Acta

110

pp. 73-78

Full Text UEA Repository

(Article)

(Published)


Appia‑Ayme, C., Hall, A., Patrick, E., Rajadurai, S., Clarke, T., Rowley, G.

(2012)

ZraP is a periplasmic molecular chaperone and a repressor of the zinc-responsive two-component regulator ZraSR,

in Biochemical Journal

442

(1)

pp. 85-93

Full Text UEA Repository

(Article)

(Published)


Breuer, M., Zarzycki, P., Shi, L., Clarke, T., Edwards, M., Butt, J., Richardson, D., Fredrickson, J., Zachara, J., Blumberger, J., Rosso, K.

(2012)

Molecular structure and free energy landscape for electron transport in the decahaem cytochrome MtrF,

in Biochemical Society Transactions

40

(6)

pp. 1198-1203

Full Text UEA Repository

(Article)

(Published)


Edwards, M., Hall, A., Shi, L., Fredrickson, J., Zachara, J., Butt, J., Richardson, D., Clarke, T.

(2012)

The Crystal Structure of the Extracellular 11-heme Cytochrome UndA Reveals a Conserved 10-heme Motif and Defined Binding Site for Soluble Iron Chelates,

in Structure

20

(7)

pp. 1275-1284

Full Text UEA Repository

(Article)

(Published)


Felgate, H., Giannopoulos, G., Sullivan, M., Gates, A., Clarke, T., Baggs, E., Rowley, G., Richardson, D.

(2012)

The impact of copper, nitrate and carbon status on the emission of nitrous oxide by two species of bacteria with biochemically distinct denitrification pathways,

in Environmental Microbiology

14

(7)

pp. 1788-1800

Full Text UEA Repository

(Article)

(Published)


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Key Research Interests

My interests lie in the characterisation of enzymes that contain metal cofactors, principally enzymes involved in bacterial respiration. These include soluble cytoplasmic and periplasmic proteins, integral membrane proteins as well as extracellular membrane associated proteins that interact with the environment. In collaboration with colleagues in the Centre for Molecular and Structural Biochemistry, my research combines a broad range of biological, biochemical and biophysical techniques to explore protein-protein interactions, electron transfer between cofactors and the mechanism of enzyme catalysis.

Video

Current Research Projects 

  • Electron transport in mineral respiring Shewanella
  • Interactions between outer membrane cytochromes and mineral surfaces
  • Reverse electron transfer (bioelectrosynthesis)
  • Biochemistry of periplasmic chaperones in Salmonella.

 

Life in our Research Group

The actions of bacteria have a profound effect on the global environment, from the cycling of greenhouse gases to the availability of minerals and nutrients in the soil. We are interested in how these processes work on a molecular level through the action of enzymes expressed by the bacteria. In order to study these enzymes we employ a broad variety of techniques across the breadth of the science faculty; from spectroscopic methods such as electron paramagnetic resonance in the Biophysical suite in Chemistry to the culturing of micro-organisms in the Wolfson Fermentation Suite in Biology. Our group is based in a well furnished laboratory on floor 2 of BIO, where we share laboratory space and equipment with Prof. Richardson, Dr. Bowater, Dr. Hemmings and Dr. Watmough.


PhD Positions

Click here for current PhD opportunities in Biological Sciences. But feel free to email me to discuss projects outside these areas and alternative sources of funding.

Postdocs & Fellows

I am always happy to discuss possibilities for postdoctoral work and collaborations. Possible funding routes include applying for fellowships, e.g. EU Marie Curie fellowships, with me acting as sponsor, or grant applications with you as a named postdoc.

Teaching Interests

  • Microbial biotechnology (BIO-6004A) (Module organiser)
  • Molecular Enzymology in Biology and Medicine (BIO-6001A)
  • Biochemistry (BIO-5002A)
  • Microbiology (BIO-5015B)
  • Physical and Analytical Methods in the Biolmolecular Sciences (BIO-4007Y)
  • Skills for Biologists (BIO-4008Y)

External Activities and Indicators of Esteem

  • Member of the Biochemical society
  • Member of the Society for General Microbiology.
  • Invited speaker at ‘Enzymology and Ecology of the Nitrogen Cycle’ (Birmingham 2010)
  • Invited speaker for Royal Society ‘Organohalide respiration’ conference (Kavli centre, 2011)
  • Invited speaker at Goldschmidt biogeochemical conference (Prague, 2011)

Administrative Posts

  • Deputy Director of ‘Biotechnology for a sustainable future’ MSc course
  • Module Organiser of BIO-M101: Microbial and Enzyme technology
  • Natural Sciences Admissions Director.