Biography

Research

Cardiovascular disease kills around 180,000 people in UK each year with around 88,000 deaths attributed to coronary heart disease and 49,000 to stroke.  Our overarching research goal is to understand the cellular and molecular processes that contribute to cardiovascular disease, particularly those that drive vascular diseases such as atherosclerosis.  As an early career scientist I trained in the laboratories of Professor David Beech FMedSci (Leeds) and Professor R. Alan North FRS (Manchester), and established an independent research group following the award of a prestigious BBSRC David Phillips Fellowship.  We collaborate with clinicians at the Norfolk & Norwich University Hospital, partners in Pharmaceutical industry and other academic groups to tackle pressing and challenging projects.  Our current work focuses on the role of signalling chemokine peptides and extracellular nucleotides in leukocyte infiltration of arteries and the early onset of atherosclerosis; cellular signalling controlling adipocyte function and inflammation; and a drug discovery programme to identify chemical modulators of P2X4 receptor activity which controls arterial tone and leukocyte activation. 

Our research is supported by the British Heart Foundation, BBSRC, Wellcome Trust, The Physiological Society and the John & Pamela Salter Trust.

Teaching & Career Development

I lecture at Masters and Undergraduate level on topics of cellular signalling, human physiology, investigation of human disease and pharmacology.  I am the Director of Employability for the School of Biological Sciences and a mentor of the Physiological Society (London).

Administration

I am a member of the Medical Research Council Review committee, SCI Faculty Employability Executive, BIO Executive, BIO Teaching Executive and BIO Staff Student Liaison Committee.  Externally I am an Editor of Purinergic Signalling.

Engagement

I am a BBSRC Regional Schools Champion currently working with secondary schools in Norfolk and Suffolk.  We have also worked with the BBC and press to disseminate our research to the general public.

Career History

  • Senior Lecturer in Pharmacology, University of East Anglia (2015 - present)
  • Lecturer in Pharmacology, University of East Anglia (2013 - 2015)
  • BBSRC David Phillips Fellow, University of East Anglia (2010 – 2013)
  • BBSRC David Phillips Fellow, University of Leeds (2008 – 2010)
  • Wellcome Trust Research Associate, University of Manchester (2004 – 2008)
  • MRC-funded PhD, University of Leeds (2000 – 2004)
  • BSc (Hons) Pharmacology, University of Leeds (1997 – 2000)

 

ResearcherID 

http://www.researcherid.com/rid/C-2142-2011

All Publications

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Layhadi, J. A., Turner, J., Crossman, D., Fountain, S. J.

(2018)

ATP Evokes Ca2+ Responses and CXCL5 Secretion via P2X4 Receptor Activation in Human Monocyte-Derived Macrophages,

in Journal of Immunology

200

(3)

pp. 1159-1168

Full Text UEA Repository

(Article)

(Published)


Layhadi, J., Fountain, S.

(2017)

P2X4 Receptor-Dependent Ca2+ Influx in Model Human Monocytes and Macrophages,

in International Journal of Molecular Sciences

18

(11)

article no. 2261

Full Text UEA Repository

(Article)

(Published)


Layhadi, J. A., Fountain, S. J.

(2017)

Influence of ER leak on resting cytoplasmic Ca2+ and receptormediated Ca2+ signalling in human macrophage,

in Biochemical and Biophysical Research Communications

487

(3)

pp. 633–639

Full Text UEA Repository

(Article)

(Published)


Stokes, L., Layhadi, J. A., Bibic, L., Dhuna, K., Fountain, S. J.

(2017)

P2X4 receptor function in the nervous system and current breakthroughs in pharmacology,

in Frontiers in Pharmacology

8

article no. 291

Full Text UEA Repository

(Article)

(Published)


Fountain, S., Sivaramakrishnan, V.

(2015)

Evidence for Extracellular ATP as a Stress Signal in a Single-Celled Organism,

in Eukaryotic Cell

14

(8)

pp. 775-782

Full Text UEA Repository

(Article)

(Published)


Campwala, H., Sexton, D. W., Crossman, D. C., Fountain, S. J.

(2014)

P2Y6 receptor inhibition perturbs CCL2-evoked signalling in human monocytic and peripheral blood mononuclear cells,

in Journal of Cell Science

127

(22)

pp. 4964-4973

Full Text UEA Repository

(Article)

(Published)


Fountain, S. J.

(2013)

Primitive ATP-activated P2X receptors: discovery, function and pharmacology,

in Frontiers in Cellular Neuroscience

7

article no. 247

Full Text UEA Repository

(Article)

(Published)


Campwala, H., Fountain, S. J.

(2013)

Constitutive and agonist stimulated ATP secretion in leukocytes,

in Communicative and Integrative Biology

6

(3)

pp. e23631

Full Text UEA Repository

(Article)

(Published)


Sivaramakrishnan, V., Fountain, S.

(2013)

Intracellular P2X receptors as novel calcium release channels and modulators of osmoregulation in Dictyostelium: A comparison of two common laboratory strains,

in Channels

7

(1)

pp. 43-46

Full Text UEA Repository

(Article)

(Published)


Sivaramakrishnan, V., Bidula, S., Campwala, H., Katikaneni, D., Fountain, S. J.

(2012)

Constitutive lysosome exocytosis releases ATP and engages P2Y receptors in human monocytes,

in Journal of Cell Science

125

(19)

pp. 4567-4575

Full Text UEA Repository

(Article)

(Published)


Li, J., Fountain, S.

(2012)

Fluvastatin suppresses native and recombinant human P2X4 receptor function,

in Purinergic Signalling

8

(2)

pp. 311-316

Full Text UEA Repository

(Article)

(Published)


Sivaramakrishnan, V., Fountain, S.

(2012)

A Mechanism of Intracellular P2X Receptor Activation,

in Journal of Biological Chemistry

287

(34)

pp. 28315-28326

Full Text UEA Repository

(Article)

(Published)


Fountain, S. J.

(2010)

Neurotransmitter Receptor Homologues of Dictyostelium discoideum,

in Journal of Molecular Neuroscience

41

(2)

pp. 263-266

Full Text UEA Repository

(Article)

(Published)


Cheong, A., Fountain, S. J., Beech, D. J.

(2009)

Quantitative RT-PCR Methods for Investigation of Low Copy Potassium Channel Gene Expression in Native Murine Arteries,

in Potassium Channels

491

(I)

pp. 19-33

Full Text UEA Repository

(Article)

(Published)


Fountain, S. J., Burnstock, G.

(2009)

An evolutionary history of P2X receptor,

in Purinergic Signalling

5

(3)

pp. 269-272

Full Text UEA Repository

(Article)

(Published)


Young, M., Fisher, J., Fountain, S., Ford, R., North, A., Khakh, B.

(2008)

Molecular Shape, Architecture, and Size of P2X4 Receptors Determined Using Fluorescence Resonance Energy Transfer and Electron Microscopy,

in The Journal of Biological Chemistry

283

(38)

pp. 26241-26251

Full Text UEA Repository

(Article)

(Published)


Fountain, S., Cao, L., Young, M., North, A.

(2008)

Permeation Properties of a P2X Receptor in the Green Algae Ostreococcus tauri,

in The Journal of Biological Chemistry

283

pp. 15122-15126

Full Text UEA Repository

(Article)

(Published)


Cao, L., Young, M. T., Broomhead, H. E., Fountain, S. J., North, A. R.

(2007)

Thr339-to-Serine Substitution in Rat P2X2 Receptor Second Transmembrane Domain Causes Constitutive Opening and Indicates a Gating Role for Lys308,

in The Journal of Neuroscience

27

(47)

pp. 12916-12923

Full Text UEA Repository

(Article)

(Published)


Fountain, S. J., Cheong, A., Li, J., Dondas, N. Y., Zeng, F., Wood, I. C., Beech, D. J.

(2007)

Kv1.5 potassium channel gene regulation by Sp1 transcription factor and oxidative stress,

in AJP - Heart and Circulatory Physiology

293

(5)

pp. 2719-2725

Full Text UEA Repository

(Article)

(Published)


Fountain, S. J., Parkinson, K., Young, M. T., Cao, L., Thompson, C. R. L., North, A. R.

(2007)

An intracellular P2X receptor required for osmoregulation in Dictyostelium discoideum,

in Nature

448

pp. 200-203

Full Text UEA Repository

(Article)

(Published)


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Key Research Interests

Our overarching research interests are the cellular and molecular mechanisms that underlie cardiovascular disease with an emphasis of artherosclerosis and vascular inflammation.  We have a particular interest in signalling cues that generate calcium signals in leukocytes and how such signals regulate process of leukocyte migration and targeting, adhesion to vascular endothelium and secretion of pro- and anti-inflammatory cytokines.  Examples of current projects include deciphering chemokine and nucleotide evoked calcium signalling in monocytes and their relationship to cardiovascular disease, and understanding how statins produce anti-inflammatory effects.  We employ an array of cutting edge techniques including high-throughput calcium imaging, patch-clamp electrophysiology, time-lapse microscopy, virus production and virus-mediated gene delivery, primary blood cell isolation and compound screening to address these research questions.

Our research is generously funded by the British Heart Foundation, BBSRC and industrial partners.

 

Research Group Membership

Samuel J. Fountain BSc PhD (Principal Investigator)

 

Research Associates


David Richards BSc MRes PhD (Senior Research Associate)

Maria del Carmen Gonzalez-Montelongo BSc PhD (Senior Research Associate)

 

Postgraduate Research Students

 

PhD Students 

Izzuddin Ahmad BSc MSc (Malaysian Government Funded Studentship) (2014 - 2018

Seema Ali  BSc (BBSRC DTP Studentship) (2014 - 2018)

Jessica Meades BSc (BBSRC iCASE Studentship with Merck, USA) (2018 - 2020)

Anna Fortuny Gomez (BBSRC DTP Studentship) (2018 - 2020)

 

 

Masters by Research

Sean Cullum BSc

 

PhD Positions

Click here for current PhD opportunities in Biological Sciences. But feel free to email me to discuss projects outside these areas and alternative sources of funding.

External Activities and Indicators of Esteem

  • The British Pharmacological Society (Member)
  • The Physiological Society, London (Member + Mentor)
  • Purinergic Signalling (Editor)

Enterprise and Engagement Activities

  • BBSRC Regional Schools Champion (2013 - 2014)

Professional Activities

  • Editor Purinergic Signalling (2013 - present)
  • Physiological Society (London) Mentor (2002 - present)
  • British Pharmacological Society (2005 - present)

Administrative Posts

  • Director of Biomedical Research Centre