Anastasia obtained her B.Sc. (Hons) degree in Pharmacology from the University of Sunderland with a Sandwich year (in respiratory pharmacology) at Bayer PLC in 1998. Her PhD in cell biology and cell physiology took place at the University of East Anglia (2002) in the laboratory of Jelena Gavrilovic, and investigated the role of growth factor and cell-matrix interactions in alveolar epithelial wound healing.
Anastasia’s research career has been focussed in the biomedical area. Her postdoctoral positions (2002-2009) were undertaken in the Department of Respiratory Medicine at the University of Cambridge in the laboratories of Edwin Chilvers and Nicholas Morrell, where she investigated the role of granulocytes in lung inflammation and asthma, and receptor trafficking in pulmonary arterial hypertension.
In 2009 she worked at the Institute of Food Research, Norwich within the field of gut mucosal immunology. Here she aimed to understand how gut epithelial stem cells are regulated by the innate immune system during homeostasis and inflammatory bowel disease.
In October 2016 Anastasia joined the School of Pharmacy at the University of East Anglia as a Lecturer in Pharmacology.
Secretory Leukoprotease Inhibitor (Slpi) Expression Is Required for Educating Murine Dendritic Cells Inflammatory Response Following Quercetin Exposure,
article no. 706Full Text UEA Repository
IL-6 Signaling Regulates Small Intestinal Crypt Homeostasis,
in Journal of Immunology
pp. 304-311Full Text UEA Repository
Luminal Microbes Promote Monocyte-Stem Cell Interactions Across a Healthy Colonic Epithelium,
in Journal of Immunology
pp. 439-451Full Text UEA Repository
Canonical Wnt signals combined with suppressed TGFβ/BMP pathways promote renewal of the native human colonic epithelium,
pp. 610-621Full Text UEA Repository
Distinct responses to hypoxia in subpopulations of distal pulmonary artery cells contribute to pulmonary vascular remodeling in emphysema,
in Pulmonary Circulation
pp. 241-249Full Text UEA Repository
Effects of the cyclin-dependent kinase inhibitor R-roscovitine on eosinophil survival and clearance,
in Clinical & Experimental Allergy
pp. 673-687Full Text UEA Repository
Failure of bone morphogenetic protein receptor trafficking in pulmonary arterial hypertension: potential for rescue,
in Human Molecular Genetics
pp. 3180-3190Full Text UEA Repository
Mutations in Bone Morphogenetic Protein Type II Receptor Cause Dysregulation of Id Gene Expression in Pulmonary Artery Smooth Muscle Cells: Implications for Familial Pulmonary Arterial Hypertension,
in Circulation Research
pp. 1212-1221Full Text UEA Repository
Eotaxin-1/CC Chemokine Ligand 11: A Novel Eosinophil Survival Factor Secreted by Human Pulmonary Artery Endothelial Cells,
in Journal of Immunology
pp. 1264-1273Full Text UEA Repository
Aminopeptidase N (CD13) Regulates Tumor Necrosis Factor-α-induced Apoptosis in Human Neutrophils,
in Journal of Biological Chemistry
pp. 12458-12467Full Text UEA Repository
Hypoxia-induced neutrophil survival is mediated by HIF-1α–dependent NF-κB activity,
in Journal of Experimental Medicine
article no. 105Full Text UEA Repository
Mechanism of cicaprost-induced desensitization in rat pulmonary artery smooth muscle cells involves a PKA-mediated inhibition of adenylyl cyclase,
in AJP: Lung Cellular and Molecular Physiology
pp. L352-L359Full Text UEA Repository
Characterization of the survival effect of tumour necrosis factor-α in human neutrophils,
in Biochemical Society Transactions
pp. 456-460Full Text UEA Repository
New insights into oxygen sensing at a cellular level,
pp. 90-92Full Text
Key Research Interests
The long-standing research interest of this lab is the study of mucosal tissue, in particular that of the lung and the gut during health and inflammation. Experimentally we use an integrated approach; our research traverses epithelial stem cells, the innate immune system and commensal bacteria.
The key to a healthy mucosa is the maintenance of the epithelial barrier, which takes place by the division of epithelial stem cells, their differentiation into different epithelial cell types and the renewal of the epithelium. During homeostasis the epithelium forms a tight barrier preventing direct contact of the external environment i.e. microbes with underlying immune cells so preventing an inflammatory response.
Our research (Skoczek et al. 2014) has shown that immune cells underlying the epithelium are critical regulators of homeostasis in the gut. Using gut organotypic culture we have shown that in health the intact crypt epithelial barrier first detects any changes in bacterial composition of the gut lumen and then rapidly recruits underlying Ly6C+ monocytes (via the MyD88 signalling pathway) from the smooth muscle and submucosal layers to the crypt epithelial stem cell niche. This physical repositioning of monocytes is significant because it causes temporary alterations in the rate renewal of the epithelium thus allowing fine tuning of immune responses to maintain health and barrier function. We have also shown that monocytes help maintain the number of crypt epithelial stem cells in vivo; further supporting our hypothesis that immune-epithelial interactions are important in the maintenance of tissue homeostasis.
During inflammation the epithelial barrier is compromised, which allows bacteria to interact directly with the body’s immune system and an inflammatory response occurs. Our hypothesis is that the highly regulated homeostatic interaction between monocytes and epithelial stem cells we observed is dysregulated during inflammation.
Homeostasis is a tightly regulated process requiring finely-tuned complex interactions between different cell types, growth factors / cytokines and their receptors. Another hypothesis in my lab is that the signaling pathways of these factors also play a role in stem cell driven tissue renewal during homeostasis or inflammation and that these factors may be epithelial-derived (autocrine) or immune cell-derived (paracrine).
Recent work published also in the Journal of Immunology has demonstrated a previously unidentified role for autocrine IL-6 signaling in the maintenance of the small intestinal crypt stem cell niche, through the differential expression of the IL-6 receptor and downstream STAT3 signaling in Paneth cells and the Wnt signaling pathway.
PAST GROUP MEMBERS
Dagmara Skczoek (former PhD student)
Petr Walcyzsko (Postdoc)
Andy Goldson (Postdoc)
CURRENT GROUP MEMBERS
Vicki Jeffery (current PhD student)