Senior Lecturer in Eukaryotic Microbiology & Course Director, MRes in Health Research
in Eukaryotic Microbiology & Course Director, MRes in Health Research
K dot Tyler at uea dot ac dot uk
Protozoan parasites cause many of the world's worst diseases and are amongst the greatest sources of suffering and death. Because most are restricted to the developing world, researching the biology of these organisms with a view to deriving effective drugs, vaccines and diagnostics has been long neglected. My research has been focussed on this group of organisms for many years and my group's research investigates the interactions between these organisms and their human host, and whether by elucidating and disrupting these interactions we can provide novel therapies.
In the fight against disease, I believe it is important that tomorrow's doctors are able to incorporate understanding and indeed practice of biomedical research into their treatment of disease; to understand the importance of the evidence base for the practice of modern medicine. For this reason at UEA I have worked to introduce a research project into the MBBS syllabus and an intercalated MRes course for those medical students wishing to pursue research actively during their medical careers. I hope to be able to offer an intercalated PhD course to medical students in the future.
For my webpages click here
For my publications click here
We are investigating the way in which Trypanosoma cruzi is able to manipulate host cardiomyoctes to facilitate invasion and for intercellular survival. Our approach is primarily by real-time fluorescence microscopy of host/fluorescent protein chimaeras and fluorescent probes and by use of molecular and chemical intervention.
Trypanosome Cell Biology
We are investigating the requirements and mechanism of cytological change in trypanosomes; in particular, the roles of cytoskeleton and membrane rafts.
We are investigating the evolution of parasite molecules which are associated with host specificity and virulence in a number of parasites.
We thank the Wellcome Foundation, The European Commission (http://www.helmholtz-hzi.de/healthy_water/), CAPES CNPq, John and Pamela Salter Trust, The Wolfson Foundation and the University of East Anglia for support.
Laboratory Web Pages
For more on what we do visit our Lab web pages
For our publications click here
PhD student "Host cell response to invasion by Trypanosoma cruzi"
PhD student "Virulence factors of anthroponotic Cryptosporidium"
Research Technician - "Establishing function of a MORN domain protein of Leishmania"
Post Doctoral Fellow "Host/parasite interaction with Trypanosoma cruzi"
Laboratory and Floor Manager
Visiting Scientist "role of parasite surface determinants in pathogenicity of chagas disease"
NERC student - Genetic heterogeneity of Avian Malaria
Visiting professor (UFRJ, Brazil) "Visualization of host cytoskeletal response during invasion by Trypanosoma cruzi"
Visiting scientist (UFSC, Brazil) "Molecular heterogeneity of Trichomonas gallinae"
Wellcome vacation scholar "Molecular Epidemiology of pathogenic protozoa in a closed avian population"
I am interested in working to ensure that clinicians are able to understand the scope and limitations of scientific research and are able to integrate research into their clinical careers. To this end I have manage provision of the short research project of the MB/BS and the intercalated MRes degree (the first of its kind in the UK). In the near future I hope to be able to offer some students the opportunity to pursue a seven year fully integrated MB/PhD program which at this time will be unique in the UK.
MRes Health Research/ MBPhD/ Intercalation
BSc Biological Sciences/ BioMedicine
Machado, FS, Tyler, KM, Brant, F, Esper, L, Teixeira, MM and Tanowitz, HB (2012) Pathogenesis of Chagas disease: time to move on. Frontiers in Biosciences, E4. pp. 1743-1758.
Lawson, B, Cunningham, AA, Chantrey, J, Hughes, LA, John, SK, Bunbury, N, Bell, D and Tyler, KM (2011) A clonal strain of Trichomonas gallinae is the aetiologic agent of an emerging avian epidemic disease. Infection, Genetics and Evolution, 11 (7). pp. 1638-1645. ISSN 15671348
Wheeler, G and Tyler, KM (2011) Widefield microscopy for live imaging of lipid domains and membrane dynamics. BBA-Biomembranes, 1808. pp. 634-641.
Yoshida, N, Tyler, KM and Llewellyn, MS (2011) Trypanosoma cruzi joins the emerging food-borne parasites. Trends in Parasitology, 27. pp. 459-466.
Bouzid, M, Heavens, D, Elwin, K, Chalmers, RM, Hunter, PR and Tyler, KM (2010) Whole Genome Amplification (WGA) for archiving and genotyping of clinical isolates of Cryptosporidium species. Parasitology, 137 (1). pp. 27-36.
Bouzid, M, Tyler, KM, Christen, R, Chalmers, RM, Elwin, K and Hunter, PR (2010) Multilocus analysis of Cryptosporidium provides evidence for ecological adaption driving genetically divergent subpopulations. BMC Microbiology, 10. p. 213.
Grisard, EC, Stoco, PH, Wagner, G, Sincero, TC, Rotava, G, Rodrigues, JB, Snoeijer, CQ, Koerich, LB, Sperandio, MM, Bayer-Santos, E, fragoso, SP, Goldenberg, S, Triana, O, Vallejo, GA, Tyler, KM, Davila, AM and Steindel, M (2010) Transcriptomic analyses of the avirulent trypanosome Trypanosoma rangeli. Molecular and Biochemical Parasitology, 174 (1). pp. 18-25.
Robinson, RA, Lawson, B, Toms, MP, Peck, KM, Kirkwood, JK, Chantrey, J, Clatworthy, MR, Evans, AE, Hughes, L, Hutchinson, C, John, S, Pennycott, T, Perkins, M, Rowley, P, Simpson, VR, Tyler, KM and Cunningham, AA (2010) Emerging Infectious Disease leads to Rapid Population Decline of Common British Birds. PLoS ONE, 5 (8). e12215.
Tyler, K.M. (2010) Book Review of "Anaerobic Parasitic Protozoa: Genomics and Molecular Biology" by C. G. Clark, P. J. Johnson and R. D. Adam. Parasites & Vectors, 3 (1). p. 45. ISSN 1756-3305
Tyler, KM, Wagner, GK, Wu, Q and Huber, KT (2010) Functional significance may underlie the taxonomic utility of single amino acid substitutions in conserved proteins. Journal of Molecular Evolution, 70 (4). pp. 395-402.
Almeida, CR, Stoco, PH, Wagner, G, Sincero, TC, Rotava, G, Bayer-Santos, E, Rodrigues, JB, Sperandio, MM, Maia, AA, Ojopi, EP, Zaha, A, Ferreira, HB, Tyler, KM, Davila, AM, Grisard, EC and Dias-Neto, E (2009) Transcriptome analysis of Taenia solium cysticerci using Open reading Frame ESTS (ORESTES). Parasites and Vectors, 2 (1). p. 35.
Tyler, KM (2009) Book Review of "Molecular Mechanisms of Parasite Invasion" by Barbara A.Burleigh and Dominique Soldati-Favre. Parasites and Vectors, 2 (1). p. 24.
Tyler, KM, Fridberg, A, Torriela, KM, Olsen, CL, Cieslak, JA, Hazlett, TL and Engman, DM (2009) Flagellar membrane association via interaction with lipid rafts. Journal of Cell Science, 122 (6). pp. 859-865.
Vijayan, S and Tyler, K (2009) Diplomatosis - Masters in Health Research (MRes). British Medical Journal (careers), bmj.b27.
Villalta, F, Scharfstein, J, Ashton, AW, Tyler, KM, Guan, F, Mukherjee, S, Lima, MF, Alvarez, S, Weiss, LM, Huang, H, Machado, FS and Tanowitz, HB (2009) Perspectives on the Trypanosoma cruzi-host cell receptor interaction. Parasitology Research, 104 (6). pp. 1251-60.
Bouzid, M, Steverding, DJ and Tyler, KM (2008) Detection and surveillance of waterborne protozoan parasites. Current Opinion in Biotechnology, 19 (3). pp. 302-306.
Fridberg, A, Olson, CL, Nakayasu, ES, Tyler, KM, Almeida, IC and Engman, D (2008) Sphingolipid synthesis is necessary for kinetoplast segregation and cytokinesis in Trypanosoma brucei. Journal of Cell Science, 121 (4). pp. 522-535.
Moraes, MH, Guarneria, AA, Girardia, FP, Rodriguesa, JB, Egera, I, Tyler, KM, Steindel, M and Grisard, EC (2008) Different serological cross-reactivity of Trypanosoma rangeli forms in Trypanosoma cruzi-infected patients sera. Parasites and Vectors, 1 (1). p. 20.
Tyler, KM (2008) Book Review of "Leishmania - after the Genome" by Peter J. Myler and Nicholas Fasel. Parasites and Vectors, 1. p. 11.
Wang, X, Jobe, M, Tyler, KM and Steverding, DJ (2008) Efficacy of common laboratory disinfectants and heat on killing trypanosomatid parasites. Parasites and Vectors, 1 (1). p. 35.
Gaspar da Silva, D, Barton, E, Bunbury, N, Lunness, P, Bell, DJ and Tyler, KM (2007) Molecular Identity and Heterogeneity of Trichomonad Parasites in a Closed Avian Population. Infection, Genetics and Evolution, 7 (4). pp. 433-440.
Bunbury, N, Barton, E, Jones, CG, Greenwood, AG, Tyler, KM and Bell, DJ (2007) Avian blood parasites in an endangered columbid: Leucocytozoon marchouxi in the Mauritian Pink Pigeon Columba mayeri. Parasitology, 134 (6). pp. 797-804.
Tyler, KM (2006) Peer-level multiple source feedback for fitness to practice. Medical Education, 40 (5). pp. 482-483.
Croxford, J, Wang, K, Miller, S, Engman, D and Tyler, KM (2005) Effects of cannabinoid treatment on Chagas disease pathogenesis: balancing inhibition of parasite invasion and immunosuppression. Cellular Microbiology, 7 (11). pp. 1592-1602.
Steverding, D and Tyler, KM (2005) Novel antitrypanosomal agents. Expert Opin Investig Drugs, 14 (8). pp. 939-955.
Tyler, KM, Murphy, SC and Engman, DM (2005) Responsive microtubule dynamics promote cell invasion by Trypanosoma cruzi. Cellular Microbiology, 7 (11). pp. 1579-1591.
Tyler, KM (2003) Maintainence of parasitaemia - is it to die for? Kinetoplastid Biology and Disease, 2 (1). p. 2.
Davila, AM, Ganguli, A and Tyler, KM (2002) Kinetoplastid Internet Symposium: A KISs for 2002. Trends Parasitol, 18 (6). p. 240.
Davila, AM and Tyler, KM (2002) Combating Kinetoplastid Diseases. Kinetoplastid Biology and Disease, 1 (1). p. 6.
Davila, AM, Ganguli, A and Tyler, KM (2001) Third Internet Conference on Salivarian Trypanosomes and Trypanosomatids. International Journal of Parasitology, 31. v-vi.
Davila, AM and Tyler, KM (2001) The net closes on trypanosomiasis. Memorias de Instituto Oswaldo Cruz, 96 (7). pp. 1025-1028.
Tyler, KM and Engman, DM (2001) The Life cycle of Trypanosoma cruzi revisited. International Journal of Parasitology, 31. pp. 472-481.
Tyler, KM, Higgs, P, Matthews, KR and Gull, K (2001) Limitation of Trypanosoma brucei parasitaemia results from a combination of density-dependent parasite differentiation and parasite killing by the host immune response. Proceedings of the Royal Soc: B Biol Sci, 268 (1482). pp. 2235-2243.
Tyler, KM, Matthews, KR and Gull, K (2001) Anisomorphic cell division by African trypanosomes. Protist, 152 (4). pp. 367-378.
Tyler, KM and Engman, DM (2000) Flagellar elongation induced by glucose limitation is preadaptive for Trypanosoma cruzi differentiation. Cyberpsychology and Behavior, 46. pp. 269-278.
Tyler, KM (1998) Differentiation and division of Trypanosoma brucei in the mammalian bloodstream. University of Manchester.
Tyler, KM, Matthews, KR and Gull, K (1997) The bloodstream differentiation - division of Trypanosoma brucei studied using mitochondrial markers. Proceedings of the Biol Sci, 264 (1387). pp. 1481-1490.
Sanchez, MJ, Tyler, KM, Rozengurt, N and Lida, J (1991) Comparison of a PCR-based diagnostic assay for Mycoplasma pulmonis with traditional detection techniques. Lab Animal, 2. pp. 249-256.
Steverding, D, Tyler, KM and Grisard, EC (2012) Delivering on promise? The Impact of Kinetoplastid Genomics on Sleeping Sickness, Chagas Disease and Leishmaniasis. In: Genomics Applications and the Developing World. UNSPECIFIED. (In Press)
Tyler, KM (2002) Preface. In: American Trypanosomiasis. Kluwer Academic Publishers. ISBN 1402073232
Tyler, KM and Engman, DM (2002) The Life-cycle of Trypanosoma cruzi. In: American Trypanosomiasis. Kluwer Academic Publishers, Boston, pp. 1-11. ISBN 1402073232
Al-Naimi, Suha, Winpenny, John P, Hunter, Paul R and Tyler, Kevin (2010) A soluble mediator from Giardia Lamblia disrupts human gastrointestinal epithelial cell function. In: British Society for Parasitology, Cardiff 29th March - 2nd April 2010.
Tyler, KM and Miles, MM (2002) American Trypanosomiasis (World Class Parasites Vol 7). Kluwer Academic Publishers. ISBN 1402073232
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