Biography

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Fraser obtained a BSc in Chemistry from the University of Sheffield in 1987 and a Dr. rer. nat. from the Department of Chemistry at the Free University of Berlin (Germany) in 1993. He pursued post-doctoral research at the Technical University Berlin (Germany) (1993-1995), the Commissariat de l’Energie Atomique, Saclay, France (1995-1998) and the Johann Wolfgang Goethe-University Frankfurt (Germany) (1998-2006).

Fraser is currently a Reader in the School of Chemistry at the University of East Anglia, Norwich where he joined the faculty in 2007. To coincide with his appointment Fraser has won a Royal Society Wolfson Research Merit Award. He leads biological EPR research in the Henry Welcome Laboratory.

His present research aims to elucidate structure / function / dynamics relationships in biomacromolecules and macromolecular complexes. Particular emphasis being on the application, and expansion, of electron paramagnetic resonance methods to study membrane proteins.

Selected publications
Refined Distances Between Paramagnetic Centers of a Multi-Copper Nitrite Reductase Determined by Pulsed EPR (iDEER) Spectroscopy
J. van Wonderen, D. N. Kostrz, C. Dennison, F. MacMillan,*
Angewandte Chemie Int. Ed. 52, 7, 1990–1993, 2013
DOI: 10.1002/anie.201208166

Interconversions of P and F Intermediates of Cytochrome c Oxidase from Paracoccus denitrificans.
I. van der Hocht, J. van Wonderen, F. Hilbers, H. Angerer, F. MacMillan, & H. Michel
Proc. Natl. Acad. Sci. USA, 108 10, 3964-3969, 2011
DOI: 10.1073/pnas.1100950108

Elucidating Mechanisms in Haem Copper Oxidases: The High Affinity QH Binding Site in Quinol Oxidase as studied by DONUT-HYSCORE Spectroscopy and Density Functional Theory.
F. MacMillan, S. Kacprzak, P. Hellwig, S. Grimaldi, H. Michel & M. Kaupp
Faraday Discussions, 148, 315-345 2011
DOI: 10.1039/c005149g

Resolving EPR spectra in the Cytochrome bc1 complex from Saccharomyces cerevisiae.
F. MacMillan, C. Lange, M. Bawn, & C. Hunte
Applied Magnetic Resonance 37, 685-692 2010
DOI: 10.1007/s00723-009-0077-4

Surface Chemistry, Reactivity, and Pore Structure of Porous Silicon Oxidized by Various Methods
J. Riikonen, M. Salomäki, J. van Wonderen, M. Kemell, W. Xu, O. Korhonen, M. Ritala, F. MacMillan, J. Salonen, and V.-P. Lehto
Langmuir, 28 (28), pp 10573–10583, 2012
DOI: 10.1021/la301642w

Applications of high resolution EPR to iron-sulphur proteins in electron transfer chains
in Metals in Biology: Applications of High Resolution EPR to Metalloenzymes
.
R. Cammack & F. MacMillan (Editors, Graeme R. Hanson and Lawrence J. Berliner),
Biological Magnetic Resonance Vol. 29 Springer Publishers, New York, 11-44 2010
DOI: 10.1007/978-1-4419-1139-1
 

Career History

  • 2007 –  present: Reader in Chemistry in the School of Chemistry and Director of the Henry Wellcome Unit for Biological EPR, UEA
  • 2007 – 2012: Wolfson Research Merit Award of The Royal Society
  • 1998 – 2006: Independent/Junior Group Leader Fachbereich Chemie, Biochemie & Pharmazie, J. W. Goethe Universität, Frankfurt, Germany
  • 1995 – 1998: Marie-Curie Stipendiat (of the EU)  at the CEA Saclay, Séction de Bioénergétique, CEA Saclay, Gif-sur-Yvette, France 1993 – 1995    Post-Doc in the Max-Volmer Institut of the Technische Universität Berlin, Germany
  • 1988 – 1993: Dr. rer. nat. in the Institut für Organische Chemie of the Freie Universität Berlin, Germany
  • 1984 – 1987: B.Sc. Sp. Hons. in Pure and Applied Chemistry at the University of Sheffield, UK

 

All Publications

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Watkins, D. W., Jenkins, J. M. X., Grayson, K. J., Wood, N., Steventon, J. W., Le Vay, K. K., Goodwin, M. I., Mullen, A. S., Bailey, H. J., Crump, M. P., MacMillan, F., Mulholland, A. J., Cameron, G., Sessions, R. B., Mann, S., Anderson, J. L. R.

(2017)

Construction and in vivo assembly of a catalytically proficient and hyperthermostable de novo enzyme,

in Nature Communications

8

(1)

article no. 358

Full Text UEA Repository

(Article)

(Published)


Mullen, A., Hall, J., Diegel, J., Hassan, I., Fey, A., MacMillan, F.

(2016)

Membrane transporters studied by EPR spectroscopy: structure determination and elucidation of functional dynamics,

in Biochemical Society Transactions

44

(3)

pp. 905-915

Full Text UEA Repository

(Article)

(Published)


Deplazes, E., Begg, S. L., Van Wonderen, J. H., Campbell, R., Kobe, B., Paton, J. C., MacMillan, F., McDevitt, C. A., O'Mara, M. L.

(2015)

Characterizing the conformational dynamics of metal-free PsaA using molecular dynamics simulations and electron paramagnetic resonance spectroscopy,

in Biophysical Chemistry

207

pp. 51-60

Full Text UEA Repository

(Article)

(Published)


Stockner, T., Mullen, A., Macmillan, F.

(2015)

Investigating the dynamic nature of the ABC transporters: ABCB1 and MsbA as examples for the potential synergies of MD theory and EPR applications,

in Biochemical Society Transactions

43

(5)

pp. 1023-1032

Full Text UEA Repository

(Article)

(Published)


Van Wonderen, J., McMahon, R., OMara, M., McDevitt, C., Thomson, A., Kerr, I., MacMillan, F., Callaghan, R.

(2014)

The central cavity of ABCB1 undergoes alternating access during ATP hydrolysis,

in FEBS Journal

281

(9)

pp. 2190-2201

Full Text UEA Repository

(Article)

(Published)


Van Wonderen, J., Kostrz, D., Dennison, C., MacMillan, F.

(2013)

Refined distances between paramagnetic centers of a multi-copper nitrite reductase determined by pulsed EPR (iDEER) spectroscopy,

in Angewandte Chemie International Edition

52

(7)

pp. 1990-1993

Full Text UEA Repository

(Article)

(Published)


Hilbers, F., van Wonderen, J., Lorenzen, W., Bode, H. B., MacMillan, F., Ludwig, B., Michel, H.

(2012)

The catalase activity of the aa(3) cytochrome c oxidase from Paracoccus denitrificans,

in Biochimica et Biophysica Acta - Bioenergetics

1817

pp. S108-S108

Full Text UEA Repository

(Article)

(Published)


Riikonen, J., Salomäki, M., Van Wonderen, J., Kemell, M., Xu, W., Korhonen, O., Ritala, M., MacMillan, F., Salonen, J., Lehto, V.

(2012)

Surface chemistry, reactivity, and pore structure of porous silicon oxidized by various methods,

in Langmuir

28

(28)

pp. 10573-10583

Full Text UEA Repository

(Article)

(Published)


von der Hocht, I., Van Wonderen, J., Hilbers, F., Angerer, H., Macmillan, F., Michel, H.

(2011)

Interconversions of P and F intermediates of cytochrome c oxidase from Paracoccus denitrificans,

in Proceedings of the National Academy of Sciences of the United States of America (PNAS)

108

(10)

pp. 3964-3969

Full Text UEA Repository

(Article)

(Published)


Macmillan, F., Kacprzak, S., Hellwig, P., Grimaldi, S., Michel, H., Kaupp, M.

(2011)

Elucidating mechanisms in haem copper oxidases: The high-affinity Q(H) binding site in quinol oxidase as studied by DONUT-HYSCORE spectroscopy and density functional theory,

in Faraday Discussions

148

pp. 315-344

Full Text UEA Repository

(Article)

(Published)


Macmillan, F., Hunte, C.

(2010)

Quinone binding and catalysis,

in Biochimica et Biophysica Acta - Bioenergetics

1797

(12)

pp. 1841

Full Text UEA Repository

(Article)

(Published)


Macmillan, F., Van Wonderen, J., Cheesman, M., Van Der Hocht, I., Angerer, H., Gennis, R., Michel, H.

(2010)

Using EPR up close and from afar: Elucidating mechanisms in haem copper oxidases,

in Biochimica et Biophysica Acta - Bioenergetics

1797

pp. 97

Full Text UEA Repository

(Article)

(Published)


von der Hocht, I., van Wonderen, J. H., MacMillan, F., Michel, H.

(2010)

Interconversions of P and F intermediates of cytochrome c oxidase from Paracoccus denitrificans,

in Biochimica et Biophysica Acta - Bioenergetics

1797

pp. 102-102

Full Text UEA Repository

(Article)

(Published)


Macmillan, F., Lange, C., Bawn, M., Hunte, C.

(2010)

Resolving the EPR Spectra in the Cytochrome bc (1) Complex from Saccharomyces cerevisiae,

in Applied Magnetic Resonance

37

(1-4)

pp. 305-316

Full Text UEA Repository

(Article)

(Published)


MacMillan, F., Cammack, R.

(2010)

Electron Magnetic Resonance of Iron-Sulfur Proteins in Electron-Transfer Chains: Resolving Complexity,

in Metals in Biology : Applications of High-Resolution EPR to Metalloenzymes.

Springer-Verlag Berlin Heidelberg

pp. 11-44

ISBN 978-1-4419-1138-4

Full Text

(Chapter (peer-reviewed))

(Published)


Michel, H., Angerer, H., Koepke, J., Macmillan, F.

(2008)

P/19 Evidence for the presence of a peroxide in the binuclear site of oxidized cytochrome c oxidase: The new catalytic cycle,

in Biochimica et Biophysica Acta - Bioenergetics

1777

(Supplement)

pp. S6-S7

Full Text UEA Repository

(Article)

(Published)


Teutloff, C., White, G., Gennis, R. B., Thomson, A. J., Macmillan, F.

(2008)

S11.8 The coupling of electron and proton transfer in haem copper oxidases as studied by peldor spectroscopy,

in Biochimica et Biophysica Acta - Bioenergetics

1777

(Supplement)

pp. S67

Full Text UEA Repository

(Article)

(Published)


Macmillan, F., Angerer, H., Kaupp, M., Teutloff, C., Michel, H.

(2008)

S11/7 The mechanism of haem copper oxidases studied by EPR spectroscopy,

in Biochimica et Biophysica Acta - Bioenergetics

1777

(Supplement)

pp. S66-S67

Full Text UEA Repository

(Article)

(Published)


Angerer, H., Macmillan, F., Michel, H.

(2008)

S11.38 The reversibility of P → F state transition in cytochrome c oxidase from Paracoccus denitrificans,

in Biochimica et Biophysica Acta - Bioenergetics

1777

pp. S74

Full Text UEA Repository

(Article)

(Published)


Wenz, T., Covian, R., Hellwig, P., Macmillan, F., Meunier, B., Trumpower, B. L., Hunte, C.

(2007)

Mutational analysis of cytochrome b at the ubiquinol oxidation site of yeast complex III,

in Journal of Biological Chemistry

282

(6)

pp. 3977-3988

Full Text UEA Repository

(Article)

(Published)


<- Page 1 of 4 ->

Key Research Interests

Current Research/Technology Expertise and Interests:

  • All Aspects of Paramagnetic Resonance Spectroscopy (EPR, ESR) including Multi-Frequency (S-, X-, Q-, W-bands) EPR and Pulsed EPR (ENDOR, PELDOR, ESEEM, HYSCORE) Spectroscopy
  • Membrane and metalloproteins including photosynthetic and respiratory proteins
  • Structure/function/dynamics relationships in biomacromolecules
  • Electron transfer and (ABC-)transporter proteins


Current Methodologies/Techniques Used

  • All modern advanced EPR spectroscopies
  • MO-calculations (DFT)
  • Freeze-quenching
  • Spin labelling


Areas for Potential Collaboration:
Anywhere where structural or structure/functional (including dynamic) information of a biomacromolecule is of importance. While endogenous spin probes (transition metal ion cofactors, organic cofactors or protein based radicals) are the probes of choice for the methods we apply, the use of exogenous spin probes (nitroxide spin labels and transition metal ions) also allows us to address inherently diamagnetic proteins and protein complexes, permitting access to similar information in these cases.

Current Research Projects:
My interests lie in the application and development of modern paramagnetic resonance spectroscopic techniques to characterise and correlate the structure and function of biomacromolecules. We apply advanced EPR techniques to study both local and global interactions of endogenous as well as exogneous spin probes providing very detailed structural information, which can be considered complementary to crystallographic and other structural techniques. In principle we are able to work with isolated proteins, protein ensembles, enriched membrane fragments, proteins reconstituted in proteoliposomes as well as in whole cells. Selected current active collaborations outside UEA include work on transporter proteins (p-Glycoprotein (Oxford), ATP-transporters (MRC Cambridge), respiratory proteins (complexes I, II, III and IV (Philadelphia, San Francisco, Freiburg, Newcastle and Frankfurt)), Colicins (York, Nottingham) and photosynthetic proteins (bioenergy systems (Tempe, Columbia)).

For Full details please see www.uea-epr.eu.

Research Funding

 

EU COST Action CM0902:
Molecular Machineries for Ion Translocation Across Biomembranes, Cellular Transport for Biotechnology
MacMillan F (Chair and Grant Holder, UEA, UK),
01 January 2010 – 17 November 2013

The Wellcome Trust
The molecular process underlying multidrug efflux by P-glycoprotein
MacMillan F, Callaghan R (University of Canberra, Australia).
January 2012 - January 2014

The John & Pamela Salter Charitable Trust
Analysis of ToIC Mutants by Electron Paramagnetic Resonance (EPR) Spectroscopy of Pulsed Electron Double Resonance (PELDOR)
Fanutti, C, MacMillan F
01 November 2010 – 30 June 2013

R17476
Synthesis of selectively spin labelled cardiolipin
MacMillan F, Hunte C (University of Freiburg, Germany)
05 February 2009 – 31 July 2013

The Royal Society; Wolfson Research Merit Award
Time domain and variable frequency EPR of membrane proteins
MacMillan F
01 April 2007 – 6 November 2012

The Biochemical Society
2011 Summer Studentship
MacMillan F
July 2011

The Royal Society; Travel Grant
52rd Annual Rocky Mountain Conference on Analytical Chemistry, Aspen, USA
MacMillan F
August 2010

The Biochemical Society Travel Grant
16th European Bioenergetics Conference – Warsaw, Poland
MacMillan F
July 2010

COST Action P15:
Advanced paramagnetic resonance methods in molecular biophysics
EU-COST Training School at the 42nd International Meeting of the RSC EPR group, Norwich
MacMillan, F
April 2009

The Wellcome Trust
The molecular mechanism of multidrug efflux by P-glycoprotein: an electron paramagnetic resonance spin label study
MacMillan F, Callaghan R (University of Oxford).
01 January 2009 - 31 July 2010

The Royal Society; Research Grant
A Q-band ENDOR investigation of ubiquinol-cytochrome c reductase (QCR)
MacMillan F
07 March 2008 – 06 July 2010

Deutsche Forschungsgemeinschaft
Multidimensional EPR-spectroscopy on protein-bound, redox-active, paramagnetic centres
MacMillan F & Prisner TP
01 January 2000 – 31 December 2005

 

 

Research Group Membership

Current PDRA's

  • Christian Teutloff (Non Stipendiary) (2007 - )
  • Jessica van Wonderen (UEA/Wellcome Trust) (2009 - )
  • Megen O’Mara (Non Stipendiary) (2012 - 2014)

PhD Students
  • Matthew Bawn (2007 - 2012)
  • Morgan Bye (2009 - 2013)

Project Students
  • Alex Morley (2012 - 2013)
  • Shivani Bhanaut (2012 - 2013)

Previous Group members
  • Ben Anderson (2011/2012)
  • Justin Bradley (Royal Society) (2012)
  • Debbie Cave (Project Student 2007/2008)
  • Zoe Karthauser (Project Student 2008/2009)
  • Glenn Tobias (Project Student 2010/2011)
  • Alicia-Anne Stratford (Project Student 2010/2011)
  • Christopher Stevens (Project Student 2010/2011)
  • Suzy Jones (PhD Student 2011)
  • Alex Morley (Biochemical Society Summer Student 2011)
     

External Activities and Indicators of Esteem

  • The Biochemist Scientific Editorial Advisory Board member - 2011 to present
  • Chair of Theme Panel  III "Bioenergetics and Metabolism" (the Biochemical Society) - 2013 to 2019
  • Scientific Committee member of the Rocky Mountains Conference on Analytical Chemistry - 2012/2013/2014
  • Chair and Grant Holder of the EU COST Action (CM0902) “Molecular machineries for ion translocation across biomembranes" - 2012 to 2013
  • Chair and Grant Holder of the EU COST Action (CM1306) “Understanding Movement and Mechanism in Molecular Machines” - 2014 to 2018
  • Treasurer of the Special Interest Group ESR of the Royal Society of Chemistry - 2010 to 2014
  • Director of Admissions for the School of Chemistry at UEA - 2009 to 2012
  • Committee Member of Theme Panel III "Bioenergetics and Metabolism" of the Biochemical Society - 2008 to present
  • Management Committee Member (UK) of the COST Action PM0902 "Molecular machineries for ion translocation across biomembranes" - 2009 to 2013
  • Associate Editor of Biochim. Biophys. Acta - 2008 to present
  • Vice-Chair of Theme Panel III "Bioenergetics and Metabolism" of the Biochemical Society - 2012 to 2013
  • Member of the Senate Commission „Nachwuchsförderung“ of the J. W. Goethe Universität. Frankfurt am Main, Deutschland - 2003 to 2007
  • Committee Member of the Special Interest Group of the Royal Society of Chemistry - 2003 to 2010

 

Administrative Posts

Chair of the School of Chemistry Web Committee