Career History

  • Senior Lecturer in Cell Biology (2004-present)
  • Lecturer in Cell Biology, University of East Anglia (1995-2004)
  • Postdoctoral Research Associate, University of East Anglia (1993-1995)
  • Ph.D. – School of Biological Sciences, University of East Anglia (1993)
  • B.Sc. - Biophysics, University of East Anglia (1989)

All Publications

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Parris, A., Williams, M.

(2015)

A human colonic crypt culture system to study regulation of stem cell-driven tissue renewal and physiological function,

in Methods in Molecular Biology

1212

pp. 141-161

Full Text UEA Repository

(Article)

(Published)


Skoczek, D. A., Walczysko, P., Horn, N., Parris, A., Clare, S., Williams, M., Sobolewski, A.

(2014)

Luminal Microbes Promote Monocyte-Stem Cell Interactions Across a Healthy Colonic Epithelium,

in Journal of Immunology

193

(1)

pp. 439-451

Full Text UEA Repository

(Article)

(Published)


Reynolds, A., Wharton, N., Parris, A., Mitchell, E., Sobolewski, A., Kam, C., Bigwood, L., El Hadi, A., Münsterberg, A., Lewis, M., Speakman, C., Stebbings, W., Wharton, R., Sargen, K., Tighe, R., Jamieson, C., Hernon, J., Kapur, S., Oue, N., Yasui, W., Williams, M.

(2014)

Canonical Wnt signals combined with suppressed TGFβ/BMP pathways promote renewal of the native human colonic epithelium,

in Gut

63

(4)

pp. 610-621

Full Text UEA Repository

(Article)

(Published)


Belshaw, N., Pal, N., Tapp, H., Dainty, J., Lewis, M., Williams, M., Lund, E., Johnson, I.

(2010)

Patterns of DNA methylation in individual colonic crypts reveal aging and cancer-related field defects in the morphologically normal mucosa,

in Carcinogenesis

31

(6)

pp. 1158-1163

Full Text UEA Repository

(Article)

(Published)


Scobioala-Laker, N., Reynolds, A., Parris, A., Mitchell, E., Lewis, M., Kennedy, H., Stebbings, W., Prior, A., Phillips, M., Beales, I., Williams, M.

(2009)

A Novel 3D Ex Vivo Model of Native Human Barrett's Oesophagus,

UEA Repository

(Paper)

(Published)


Reynolds, A., Parris, A., Evans, L., Lindqvist, S., Sharp, P., Lewis, M., Tighe, R., Williams, M.

(2007)

Dynamic and differential regulation of NKCC1 by calcium and cAMPin the native human colonic epithelium,

in The Journal of Physiology

582

(2)

pp. 507-524

Full Text UEA Repository

(Article)

(Published)


Williams, M., Spahos, T., Reynolds, A., Parris, A., Tighe, R., Speakman, C., Beales, I., Lewis, M.

(2007)

Taurodeoxycholic acid induces calcium oscillations in isolated human colonic crypts,

in Gastroenterology

132

(Suppl 2)

pp. 8

UEA Repository

(Article)

(Published)


Parris, A., Reynolds, A., Spahos, A., Munsterberg, A., Tighe, R., Lewis, M., Cook, J., Prior, A., Speakman, C., Stebbings, [. V., Hernon, J., Beales, I., Ellamin, K., Williams, M.

(2006)

Human colonic crypt renewal ex vivo.,

in Gastroenterology

130

(Suppl 2)

pp. 1677

UEA Repository

(Article)

(Published)


Parris, A., Reynolds, A., Spahos, T., Williams, M.

(2005)

Wnt signalling mobilises beta-catenin and stimulates cell proliferation in isolated human colonic crypts,

in Gastroenterology

128

(4)

pp. 489

UEA Repository

(Article)

(Published)


Reynolds, A., Addison, S., Lindqvist, S., Williams, M.

(2005)

Propagating calcium signals along human colonic crypts dynamically regulate NKCC1 phosphorylation and activity.,

in Gastroenterology

128

pp. 357-358

UEA Repository

(Article)

(Published)


Parris, A., Reynolds, A., Addison, S., Lindqvist, S., Hernon, J., Spahos, T., Tighe, T., Lewis, M., Mackay, J., Westgarth, D., Fellows, F., Bell, D., Kapur, S., Prior, A., Williams, M.

(2004)

Spatial correlation between acetylcholine-induced calcium signals and acetylcholine-induced cell proliferation along the isolated human colonic crypt axis.,

in Gastroenterology

126

pp. 140

UEA Repository

(Article)

(Published)


Addison, S., Lindqvist, S., Setchell, O., Hernon, J., Tighe, R., Rhodes, M., Stebbings, W., Williams, M.

(2003)

Top-down aberrant calcium signals in colonic crypts derived from the flat mucosa of polyp- and colorectal cancer-patients,

in Gastroenterology

124

(4, Supplement 1)

pp. A282

Full Text UEA Repository

(Article)

(Published)


Addison, S., Lindqvist, S., Setchell, O., Hernon, J., Tighe, R., Mackay, J., Rhodes, M., Stebbings, W., Williams, M.

(2003)

Prostaglandin E2 induces calcium signals in the proliferative zone of isolated human colonic crypts,

in Gastroenterology

124

(4)

pp. A276-A276

Full Text UEA Repository

(Article)

(Published)


Hernon, J., Lindqvist, S., Prior, A., Williams, M.

(2003)

Carbachol induces ERK 1/2 activation and stimulates cell proliferation via a metalloproteinase- and TGFá dependent pathway,

in Gastroenterology

124

(Suppl 1)

pp. 285

Full Text UEA Repository

(Article)

(Published)


Olano-Martin, E., Williams, M., Gibson, G. R., Rastall, R. A.

(2003)

Pectins and pectic-oligosaccharides inhibit Escherichia coliO157:H7 Shiga toxin as directed towards the human colonic cell line HT29,

in FEMS Microbiology Letters

218

(1)

pp. 101-105

Full Text UEA Repository

(Article)

(Published)


Lindqvist, S., Hernon, J., Sharp, P., Johns, N., Addison, S., Watson, M., Tighe, R., Greer, S., Mackay, J., Rhodes, M., Lewis, M., Stebbings, W., Speakman, C., Evangelista, S., Johnson, I., Williams, M.

(2002)

The colon-selective spasmolytic otilonium bromide inhibits muscarinic M(3) receptor-coupled calcium signals in isolated human colonic crypts,

in British Journal of Pharmacology

137

(7)

pp. 1134-1142

Full Text UEA Repository

(Article)

(Published)


Hernon, J., Lindqvist, S., Rhodes, M., Speakman, C., Stebbings, W., Williams, M.

(2002)

Acetylcholine stimulates Human Colonic epithelial cell growth via calcium dependent MAP Kinase activation,

in Colorectal Disease

pp. 4-41

UEA Repository

(Article)

(Published)


Hernon, J., Lindqvist, S., Rhodes, M., Speakman, C., Tighe, R., Murphy, G., Stebbings, W., Williams, M.

(2002)

Molecular mechanisms of acetylcholine-induced ERK1/2 activation in HT29 cells and isolated human colonic crypts,

in Gastroenterology

122

(4)

pp. 991-991

UEA Repository

(Article)

(Published)


Hotchkiss, A., Olano-Martin, E., Williams, M., Grace, W., Gibson, G., Rastall, R.

(2002)

Pectic oligosaccharides as prebiotics.,

in Abstracts of Papers of the American Chemical Society

223

pp. 34

UEA Repository

(Article)

(Published)


Sharp, P., Tandy, S., Yamaji, S., Tennant, J., Williams, M., Singh Srai, S. K.

(2002)

Rapid regulation of divalent metal transporter (DMT1) protein but not mRNA expression by non-haem iron in human intestinal Caco-2 cells,

in FEBS Letters

510

(1-2)

pp. 71-76

Full Text UEA Repository

(Article)

(Published)


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Key Research Interests

I am a physiologist interested in the cellular and molecular mechanisms that govern the self-renewal and physiological function of the human intestinal epithelium. A major focus of our work is to understand how age-related molecular damage and inherited genetic defects pre-dispose the intestinal epithelium to the onset of gastrointestinal disease. The ethos of our work is to conduct comparative studies on human tissue samples derived from young and old healthy subjects, and from patients with inflammatory bowel disease and cancer. To this end, our laboratory has developed an ex vivo culture model of the human intestinal epithelium that is amenable to bio-imaging and the investigation of cellular signals for crypt cell proliferation, migration, differentiation and secretion.

Current Research Projects

  • Signalling self-renewal in the human intestinal epithelium ex vivo (Collaboration with Dr Munsterberg, BIO, UEA)
  • Epigenetic modulation of Wnt signalling in the ageing human intestinal epithelium: consequences for tissue homeostasis (Collaboration with Dr Nigel Belshaw and Prof. Ian Johnson at the Institute of Food Research).
  • Wnt signaling pathways in colon cancer initiation
  • Molecular regulation of intestinal fluid secretion in health and disease.
  • Inflammatory signals for Barrett’s oesophagus (Collaboration with Mike Lewis, Norfolk and Norwich University Hospital)

 

Life in our research group 

Bio-imaging and functional genomic approaches are applied to a3D ex vivo culture model of the human colonic epithelium, which was developed in collaboration with clinicians at the Norfolk and Norwich University Hospital. The cellular and molecular mechanisms governing adult tissue homeostasis in health and disease are interrogated by utilising recombinant/pharmacological activators/inhibitors, RNAi and GFP transgenes, in combination with bright field / fluorescence time-lapse imaging and immunocytochemistry/in situ hyrbridisation. Regulation of stem cell-driven tissue renewal by Wnt signals and the mechanisms of fluid transport across the polarised intestinal epithelium are of particular interest. In a broader context, the cultured colonic crypt also serves as a model for other self-renewing tissues and polarised epithelia. Although laboratory life is somewhat crypt-centric, the resident cryptomaniacs are let out on occasion to partake in social activities such as sailing, ice-skating, dancing, BBQs and dining, a favourite restaurant being ... the Crypt!

PhD Positions 

Click here for current PhD opportunities in Biological Sciences. But feel free to email me to discuss projects outside these areas and alternative sources of funding.

Postdocs & Fellows 

I am always happy to discuss possibilities for postdoctoral work and collaborations. Possible funding routes include applying for fellowships, e.g. EU Marie Curie fellowships, Royal Society and others, or grant applications with you as a named post-doc.

Teaching Interests

  • Maths and Stats (1A6/7Y)
  • Introduction to Biomedicine (1A5Y)
  • Human Physiology (2B05)
  • Cell Biology (2B06)
  • Clinical Genetics (2B19)
  • Biomedicine (3C34)
  • Human Nutrition, Diet and Health (3C37)
  • Blood Biomedicine (3C53)
  • I offer undergraduate student research projects (3C32)

External Activities and Indicators of Esteem

  • PhD external examiner
  • Reviewer for American Journal Physiology, Journal of Biological Chemistry, FASEB etc.
  • Grant Reviewer for BBSRC, MRC etc.
  • Invited speaker: Physiology 2010; Molecular Regulation of epithelial transport protein trafficking (Manchester, 2010)
  • Invited Speaker: FASEB 2007 Summer Conference GI Tract XII: The molecular and integrative basis for GI homeostasis and disease (Colorado, 2007)
  • Plenary session speaker: American Gastroenterological Association (San Diego) 2008
  • Plenary session speaker: American Gastroenterological Association (Washington, DC) 2007
  • Plenary session speaker: British Society of Gastroenterology (Birmingham) 2006
  • Plenary session speaker: American Gastroenterological Association (Los Angeles) 2006
  • Invited speaker 11th International Conference on Ulcer Research (Dubrovnik) 2003
  • Invited speaker: XV International Symposium on Morphological Science (Kyoto) 2000
  • Member of Physiological Society

Administrative Posts

  • Member of Food and Health Research Alliance steering group
  • Module organiser for Introduction to Biomedicine (1A5Y), Clinical Genetics (2B19), Biomedicine (3C34), Blood Biomedicine (3C53)
  • University Human Tissue Safety Officer