Career History

  • 1997 - 2001 MBiochem, St-Catherine's College, Oxford University, UK
  • 2001 - 2005 DPhil, Department of Plant Sciences, Oxford University, UK
  • 2005 - 2011 Postdoctoral researcher, Biozentrum, University of Basel, CH
  • 2011 - present Synergy Lecturer in Plant and Microbial Science, UEA/JIC, UK

ResearcherID  

http://www.researcherid.com/rid/G-8319-2011

All Publications

Grenga, L., Chandra, G., Saalbach, G., Galmozzi, C. V., Kramer, G., Malone, J. G.

(2017)

Analyzing the Complex Regulatory Landscape of Hfq – an Integrative, Multi-Omics Approach,

in Frontiers in Microbiology

8

article no. 1784

Full Text UEA Repository

(Article)

(Published)


Grenga, L., Little, R. H., Malone, J. G.

(2017)

Quick change: post-transcriptional regulation in Pseudomonas,

in FEMS Microbiology Letters

364

(14)

article no. fnx125

Full Text UEA Repository

(Article)

(Published)


Campilongo, R., Fung, R. K. Y., Little, R. H., Grenga, L., Trampari, E., Pepe, S., Chandra, G., Stevenson, C. E. M., Roncarati, D., Malone, J. G.

(2017)

One ligand, two regulators and three binding sites: How KDPG controls primary carbon metabolism in Pseudomonas,

in PLoS Genetics

13

(6)

article no. e1006839

Full Text UEA Repository

(Article)

(Published)


Mauchline, T. H., Malone, J. G.

(2017)

Life in earth – the root microbiome to the rescue?,

in Current Opinion in Microbiology

37

pp. 23-28

Full Text UEA Repository

(Article)

(Published)


Kirkpatrick, C. L., Lesouhaitier, O., Malone, J. G., An, S., Caly, D. L.

(2017)

Interaction and signalling networks: a report from the fourth ‘Young Microbiologists Symposium on Microbe Signalling, Organisation and Pathogenesis’,

in Microbiology

163

(1)

pp. 4-8

Full Text UEA Repository

(Article)

(Published)


Nguyen, D., Melnik, A., Koyama, N., Lu, X., Schorn, M., Fang, J., Aguinaldo, K., Lincecum Jr, T., Ghequire, M., Carrion, V., Cheng, T., Duggan, B., Malone, J., Mauchline, T., Sanchez, L., Kilpatrick, A. M., Raaijmakers, J., De Mot, R., Moore, B., Medema, M., Dorrestein, P.

(2016)

Indexing the Pseudomonas specialized metabolome enabled the discovery of poaeamide B and the bananamides,

in Nature Microbiology

2

article no. 16197

Full Text UEA Repository

(Article)

(Published)


Pfeilmeier, S., Caly, D. L., Malone, J. G.

(2016)

Bacterial pathogenesis of plants: Future challenges from a microbial perspective,

in Molecular Plant Pathology

17

(8)

pp. 1298–1313

Full Text UEA Repository

(Article)

(Published)


Skotnicka, D., Smaldone, G. T., Petters, T., Trampari, E., Liang, J., Kaever, V., Malone, J. G., Singer, M., Søgaard-Andersen, L.

(2016)

A Minimal Threshold of c-di-GMP Is Essential for Fruiting Body Formation and Sporulation in Myxococcus xanthus,

in PLoS Genetics

12

(5)

article no. e1006080

Full Text UEA Repository

(Article)

(Published)


Pfeilmeier, S., Saur, I. M., Rathjen, J. P., Zipfel, C., Malone, J. G.

(2016)

High levels of cyclic-di-GMP in plant-associated Pseudomonas correlate with evasion of plant immunity,

in Molecular Plant Pathology

17

(4)

pp. 521-531

Full Text UEA Repository

(Article)

(Published)


Little, R. H., Grenga, L., Saalbach, G., Howat, A. M., Pfeilmeier, S., Trampari, E., Malone, J. G.

(2016)

Adaptive remodeling of the bacterial proteome by specific ribosomal modification regulates Pseudomonas infection and niche colonisation,

in PLoS Genetics

12

(2)

article no. e1005837

Full Text UEA Repository

(Article)

(Published)


Mauchline, T., Chedom-Fotso, D., Chandra, G., Samuels, T., Greenaway, N., Backhaus, A., McMillan, V., Canning, G., Powers, S. J., Hammond-Kosack, K. E., Hirsch, P. R., Clark, I. M., Mehrabi, Z., Roworth, J., Burnell, J., Malone, J. G.

(2015)

An analysis of Pseudomonas genomic diversity in take-all infected wheat fields reveals the lasting impact of wheat cultivars on the soil microbiota,

in Environmental Microbiology

17

(11)

pp. 4764-4778

Full Text UEA Repository

(Article)

(Published)


Trampari, E., Stevenson, C. E., Little, R. H., Wilhelm, T., Lawson, D. M., Malone, J. G.

(2015)

Bacterial rotary export ATPases are allosterically regulated by the nucleotide second messenger cyclic-di-GMP,

in Journal of Biological Chemistry

290

(40)

pp. 24470-24483

Full Text UEA Repository

(Article)

(Published)


Malone, J.

(2015)

Role of small colony variants in persistence of Pseudomonas aeruginosa infections in cystic fibrosis lungs,

in Infection and Drug Resistance

2015

(8)

pp. 237-247

Full Text UEA Repository

(Article)

(Published)


Caly, D., Coulthurst, S., Geoghegan, J., Malone, J., Ryan, R.

(2012)

Socializing, networking and development: a report from the second ‘Young Microbiologists Symposium on Microbe Signalling, Organization and Pathogenesis’,

in Molecular Microbiology

86

(3)

pp. 501-512

Full Text UEA Repository

(Article)

(Published)


Malone, J., Jaeger, T., Manfredi, P., Dötsch, A., Blanka, A., Bos, R., Cornelis, G., Häussler, S., Jenal, U.

(2012)

The YfiBNR Signal Transduction Mechanism Reveals Novel Targets for the Evolution of Persistent Pseudomonas aeruginosa in Cystic Fibrosis Airways,

in PLoS Pathogens

8

(6)

article no. e1002760

Full Text UEA Repository

(Article)

(Published)


Malone, J., Jaeger, T., Spangler, C., Ritz, D., Spang, A., Arrieumerlou, C., Kaever, V., Landmann, R., Jenal, U.

(2010)

YfiBNR Mediates Cyclic di-GMP Dependent Small Colony Variant Formation and Persistence in Pseudomonas aeruginosa,

in PLoS Pathogens

6

(3)

article no. e1000804

Full Text UEA Repository

(Article)

(Published)


Silby, M., Cerdeño-Tárraga, A., Vernikos, G., Giddens, S., Jackson, R., Preston, G., Zhang, X., Moon, C., Gehrig, S., Godfrey, S., Knight, C., Malone, J., Robinson, Z., Spiers, A., Harris, S., Challis, G., Yaxley, A., Harris, D., Seeger, K., Murphy, L., Rutter, S., Squares, R., Quail, M., Saunders, E., Mavromatis, K., Brettin, T., Bentley, S., Hothersall, J., Stephens, E., Thomas, C., Parkhill, J., Levy, S., Rainey, P., Thomson, N.

(2009)

Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens,

in Genome Biology

10

(5)

pp. R51

Full Text UEA Repository

(Article)

(Published)


Key Research Interests

My group studies the molecular mechanisms underlying bacterial signal transduction during responses to the environment. To achieve this we employ a range of processes including genetics, cell and molecular microbiology and biochemistry. Our current research focuses on the control of rhizosphere colonisation by the widespread Gram-negative bacterium Pseudomonas fluorescens.

Biocontrol agents; soil microorganisms that promote plant growth and/or suppress pathogens represent an attractive potential alternative to existing chemical pesticides. The commensal soil bacterium Pseudomonas fluorescens is a prominent biocontrol species that forms beneficial relationships with plants and suppresses fungal growth. The effectiveness of biocontrol is directly related to the effectiveness of bacterial rhizosphere colonisation. However, despite much research into biocontrol by P. fluorescens, the internal signalling systems within the bacteria that control root colonisation are only poorly understood. Recent work from our laboratory and others has identified an important role for the ubiquitous bacterial second messenger cyclic-di-GMP in the control of P. fluorescens root colonisation. Cyclic-di-GMP is found in almost every bacterial species on Earth, and is a critical component of the microbial decision-making machinery that controls when, where and how bacteria initiate biofilm formation, progress through the cell cycle, produce and secrete secondary metabolites or regulate motility and virulence factors.

In my previous laboratory, we used in-vitro expression technology (IVET) to identify a range of P. fluorescens genes that are specifically up-regulated in the rhizosphere. These include potential cdG targets such as the wss exopolysaccharide synthase operon, as well as several cdG-related operons. We have since shown that these rhizosphere up-regulated cdG systems control important aspects of bacterial behaviour, including attachment to plant roots and swarming motility. At least one system appears to be crucial for the effective colonisation of the wheat rhizosphere. My group is currently working to functionally characterise these cdG systems, with the overall aim of constructing an integrated molecular model for cdG signalling during the process of P. fluorescens rhizosphere colonisation.
 

Current Research Projects  

  • Functional characterisation of a central regulator of Pseudomonas fluorescens rhizosphere colonisation
  • Analysis of the cyclic-di-GMP network regulating wheat rhizosphere colonisation by Pseudomonas fluorescens.

 

PhD Positions

Click here for current PhD opportunities in Biological Sciences. But feel free to email me to discuss projects outside these areas and alternative sources of funding.

 

Teaching Interests

  • BIO-1A03 (Practical course).

External Activities and Indicators of Esteem

  • A-level photosynthesis workshop.