Biography

Studied Biochemistry at the University of Stirling and took a PhD in plant biochemistry at UCNW, Bangor. Undertook post doctoral work in the field of cryopreservation (Brighton) and inositol phosphate biochemistry (Cambridge). Won a BBSRC Advanced Research Fellowship and spent time at the Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam, Germany.

Google Scholar Profile: https://scholar.google.co.uk/citations?user=WEkkWN0AAAAJ

Career History

  • Reader, School of Biological Sciences, UEA (2016 -)
  • Senior Lecturer, School of Biological Sciences, UEA (2009 - 2016)
  • Lecturer, School of Biological Sciences, UEA (2001 - 2008)
  • British Council Visiting Researcher, Max-Planck-Institut für Molekulare Pflanzenphysiologie, Germany (1998) 
  • BBSRC Advanced Research Fellow, Dept Plant Sciences, University of Cambridge (1997-2000)
  • Postdoctoral Research Associate, Dept Plant Sciences, University of Cambridge (1990-1997)
  • Postdoctoral Researcher, Dept Pharmacy, Brighton Polytechnic (1986-1989)
  • Ph.D. University of Wales (1986)

 

Highlighted Publications

See All

Stentz, R., Osborne, S., Horn, N., Li, A., Hautefort, I., Bongaerts, R., Rouyer, M., Bailey, P., Shears, S., Hemmings, A., Brearley, C. A., Carding, S.

(2014)

A Bacterial Homolog of a Eukaryotic Inositol Phosphate Signaling Enzyme Mediates Cross-kingdom Dialog in the Mammalian Gut,

in Cell Reports

6.

pp. 646-656

Full Text UEA Repository

(Article)

(Published)


Estavillo, G., Crisp, P., Pornsiriwong, W., Wirtz, M., Collinge, D., Carrie, C., Giraud, E., Whelan, J., David, P., Javot, H., Brearley, C., Hell, R., Marin, E., Pogson, B.

(2011)

Evidence for a SAL1-PAP Chloroplast Retrograde Pathway That Functions in Drought and High Light Signaling in Arabidopsis,

in The Plant Cell

23.

pp. 3992-4012

Full Text UEA Repository

(Article)

(Published)


Gonzalez, B., Banos-Sanza, J., Villatea, M., Brearley, C., Sanz-Aparicio, J.

(2010)

Inositol 1,3,4,5,6-pentakisphosphate 2-kinase is a distant IPK member with a singular inositide binding site for axial 2-OH recognition,

in Proceedings of the National Academy of Sciences

107.

pp. 9608-9613

Full Text UEA Repository

(Article)

(Published)


Nagy, R., Grob, H., Weder, B., Green, P., Klein, M., Frelet, A., Schjoerring, J., Brearley, C., Martinoia, E.

(2009)

The Arabidopsis ATP-binding cassette protein ATMRP5/ATABCC5 is a high-affinity inositol hexakisphosphate transporter involved in guard cell signaling and phytate storage,

in Journal of Biological Chemistry

284.

pp. 33614-33622

Full Text UEA Repository

(Article)

(Published)


All Publications

See Highlights
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George, T. S., Giles, C. D., Menezes-Blackburn, D., Condron, L. M., Gama-Rodrigues, A. C., Jaisi, D., Lang, F., Neal, A. L., Stutter, M. I., Almeida, D. S., Bol, R., Cabugao, K. G., Celi, L., Cotner, J. B., Feng, G., Goll, D. S., Hallama, M., Krueger, J., Plassard, C., Rosling, A., Darch, T., Fraser, T., Giesler, R., Richardson, A. E., Tamburini, F., Shand, C. A., Lumsdon, D. G., Zhang, H., Blackwell, M. S. A., Wearing, C., Mezeli, M. M., Almås, Å. R., Audette, Y., Bertrand, I., Beyhaut, E., Boitt, G., Bradshaw, N., Brearley, C. A., Bruulsema, T. W., Ciais, P., Cozzolino, V., Duran, P. C., Mora, M. L., de Menezes, A. B., Dodd, R. J., Dunfield, K., Engl, C., Frazão, J. J., Garland, G., González Jiménez, J. L., Graca, J., Granger, S. J., Harrison, A. F., Heuck, C., Hou, E. Q., Johnes, P. J., Kaiser, K., Kjær, H. A., Klumpp, E., Lamb, A. L., Macintosh, K. A., Mackay, E. B., McGrath, J., McIntyre, C., McLaren, T., Mészáros, E., Missong, A., Mooshammer, M., Negrón, C. P., Nelson, L. A., Pfahler, V., Poblete-Grant, P., Randall, M., Seguel, A., Seth, K., Smith, A. C., Smits, M. M., Sobarzo, J. A., Spohn, M., Tawaraya, K., Tibbett, M., Voroney, P., Wallander, H., Wang, L., Wasaki, J., Haygarth, P. M.

(2017)

Organic phosphorus in the terrestrial environment: a perspective on the state of the art and future priorities,

in Plant and Soil

Full Text UEA Repository

(Article)

(E-pub ahead of print)


Rodriguez-Ramiro, I., Brearley, C., Bruggraber, S., Perfecto, A., Shewry, P., Fairweather-Tait, S.

(2017)

Assessment of iron bioavailability from different bread making processes using an in vitro intestinal cell model,

in Food Chemistry

228

pp. 91–98

Full Text UEA Repository

(Article)

(Published)


Neal, A. L., Rossmann, M., Brearley, C., Akkari, E., Guyomar, C., Clark, I. M., Allen, E., Hirsch, P. R.

(2017)

Land-use influences phosphatase gene microdiversity in soils,

in Environmental Microbiology

19

(7)

pp. 2740–2753

Full Text UEA Repository

(Article)

(Published)


Whitfield, H., Riley, A. M., Diogenous, S., Godage, H. Y., Potter, B. V., Brearley, C. A.

(2017)

Simple synthesis of 32P-labelled inositol hexakisphosphates for study of phosphate transformations,

in Plant and Soil

Full Text UEA Repository

(Article)

(E-pub ahead of print)


Franco-Echevarría, E., Sanz-Apricio, J., Brearley, C. A., González-Rubio, J. M., González, B.

(2017)

The crystal structure of mammalian inositol 1,3,4,5,6-pentakisphosphate 2-kinase reveals a new zinc-binding site and key features for protein function,

in The Journal of Biological Chemistry

292

(25)

pp. 10534-10548

Full Text UEA Repository

(Article)

(Published)


Laird, S., Brearley, C., Kuehn, I., Miller, H.

(2016)

The effect of high phytase doses on phytate degradation and myo-inositol release in the grower pig,

in Advances in Animal Biosciences

7

(1)

article no. 105

Full Text

(Abstract)

(Published)


Stentz, R., Horn, N., Cross, K., Salt, L., Brearley, C., Livermore, D. M., Carding, S. R.

(2015)

Cephalosporinases associated with outer membrane vesicles released by Bacteroides spp. protect gut pathogens and commensals against beta-lactam antibiotics,

in Journal of Antimicrobial Chemotherapy

70

(3)

pp. 701-709

Full Text UEA Repository

(Article)

(Published)


Stentz, R., Osborne, S., Horn, N., Li, A., Hautefort, I., Bongaerts, R., Rouyer, M., Bailey, P., Shears, S., Hemmings, A., Brearley, C. A., Carding, S.

(2014)

A Bacterial Homolog of a Eukaryotic Inositol Phosphate Signaling Enzyme Mediates Cross-kingdom Dialog in the Mammalian Gut,

in Cell Reports

6

(4)

pp. 646-656

Full Text UEA Repository

(Article)

(Published)


Banoz-Sanz, J., Sanz-Aparicio, J., Whitfield, H., Hamilton, C., Brearley, C., Gonzalez, B.

(2012)

Conformational Changes in Inositol 1,3,4,5,6-Pentakisphosphate 2-Kinase upon Substrate Binding: Role of N-terminal lobe and enantiomeric substrate preference,

in The Journal of Biological Chemistry

287

(35)

pp. 29237-29249

Full Text UEA Repository

(Article)

(Published)


Banos-Sanz, J., Sanz-Aparicio, J., Brearley, C., Gonzalez, B.

(2012)

Expression, purification, crystallization and preliminary X-ray diffraction analysis of the apo form of Ins5 2-K from Arabidopsis thaliana,

in Acta Crystallographica Section F

68

pp. 701-704

UEA Repository

(Article)

(Published)


Hanke, D., Parmar, P., Caddick, S., Green, P., Brearley, C.

(2012)

Synthesis of inositol phosphate ligands of plant hormone receptor complexes: pathways of inositol hexakisphosphate turnover,

in Biochemical Journal

444

(3)

pp. 601-609

UEA Repository

(Article)

(Published)


Estavillo, G., Crisp, P., Pornsiriwong, W., Wirtz, M., Collinge, D., Carrie, C., Giraud, E., Whelan, J., David, P., Javot, H., Brearley, C., Hell, R., Marin, E., Pogson, B.

(2011)

Evidence for a SAL1-PAP Chloroplast Retrograde Pathway That Functions in Drought and High Light Signaling in Arabidopsis,

in The Plant Cell

23

(11)

pp. 3992-4012

Full Text UEA Repository

(Article)

(Published)


Seipke, R., Barke, J., Brearley, C., Hill, L., Yu, D., Goss, R., Hutchings, M.

(2011)

A single Streptomyces symbiont makes multiple antifungals to support the fungus farming ant Acromyrmex octospinosus,

in PLoS ONE

6

(8)

article no. e22028

Full Text UEA Repository

(Article)

(Published)


Panzeri, D., Cassani, E., Doria, E., Tagliabue, G., Forti, L., Campion, B., Bollini, R., Brearley, C., Pilu, R., Nielsen, E., Sparvoli, F.

(2011)

A defective ABC transporter of the MRP family, responsible for the bean lpa1 mutation, affects the regulation of the phytic acid pathway, reduces seed myo-inositol and alters ABA sensitivity,

in New Phytologist

pp. no-no

Full Text UEA Repository

(Article)

(Published)


Gonzalez, B., Banos-Sanza, J., Villatea, M., Brearley, C., Sanz-Aparicio, J.

(2010)

Inositol 1,3,4,5,6-pentakisphosphate 2-kinase is a distant IPK member with a singular inositide binding site for axial 2-OH recognition,

in Proceedings of the National Academy of Sciences

107

(21)

pp. 9608-9613

Full Text UEA Repository

(Article)

(Published)


Robson, F., Okamoto, H., Patrick, E., Harris, S., Wasternack, C., Brearley, C., Turner, J.

(2010)

Jasmonate and Phytochrome - A signaling in Arabidopsis wound and shade responses are integrated through JAZ1 stability,

in Plant Cell

22

(4)

pp. 1143-1160

Full Text UEA Repository

(Article)

(Published)


Guymer, D., Maillard, J., Agacan, M., Brearley, C., Sargent, F.

(2010)

Intrinsic GTPase activity of a bacterial twin-arginine translocation proofreading chaperone induced by domain swapping,

in FEBS Journal

277

(2)

pp. 511-525

Full Text UEA Repository

(Article)

(Published)


Baños-Sanz, J., Villate, M., Sanz-Aparicio, J., Brearley, C., González, B.

(2010)

Crystallization and preliminary X-ray diffraction analysis of inositol 1,3,4,5,6-pentakisphosphate kinase from Arabidopsis thaliana,

in Acta Crystallographica Section F Structural Biology and Crystallization Communications

66

(1)

pp. 102-106

Full Text UEA Repository

(Article)

(Published)


Todd, J., Curson, A., Nikolaidou-Katsaraidou, N., Brearley, C., Watmough, N., Chan, Y., Bulman Page, P., Sun, L., Johnston, A.

(2010)

Molecular dissection of bacterial acrylate catabolism - unexpected links with dimethylsulfoniopropionate catabolism and dimethyl sulfide production,

in Environmental Microbiology

12

(2)

pp. 327-343

Full Text UEA Repository

(Article)

(Published)


Nagy, R., Grob, H., Weder, B., Green, P., Klein, M., Frelet, A., Schjoerring, J., Brearley, C., Martinoia, E.

(2009)

The Arabidopsis ATP-binding cassette protein ATMRP5/ATABCC5 is a high-affinity inositol hexakisphosphate transporter involved in guard cell signaling and phytate storage,

in Journal of Biological Chemistry

284

pp. 33614-33622

Full Text UEA Repository

(Article)

(Published)


<- Page 1 of 2 ->

Key Research Interests

Research in this laboratory addresses the biochemistry and cell biology of inositol phosphates and the enzmes that interconvert them, commonly in plants but also in animal digestive situations where plant materials are components of animal feed.   Our research is aligned closely with the needs of, and supported by, industry.  Our expertise in inositol phosphate analysis and biochemistry is applied in a number of fields ranging from germination biology, particularly malting scenarios, to human disease. We are developing novel assays of ligand binding to enzymes of inositol metabolism. Our research exploits the disciplines of enzymology and, with Dr Hemmings (BIO), structural biology. Together, we have solved structures of a number of enzymes of inositol metabolism and have developed powerful probes of enzyme function relevant to inositol phosphate signalling in disease contexts.

Current Collaborators

  • Dr. Tzyy-Jen Chiou (Agricultural Biotechnology Research Center, Academia Sinica, Taiwan)
  • Dr. Beatriz González (CSIC, Madrid)
  • Prof. Enrico Martinoia (University of Zürich)
  • Prof. Barry Potter (University of Oxford)

PhD Positions

Click here for current PhD opportunities in Biological Sciences. But feel free to email me to discuss projects outside these areas and alternative sources of funding.

External Activities and Indicators of Esteem

  • Member of Biochemical Society Theme Panel V 'Signalling' (2010 - 2015)

Enterprise and Engagement Activities

  • Plenary Speaker 2nd International Phytase Summit, Rome (December 2012)
  • Consultant to AB Vista (UK) Ltd (Marlborough, UK) and AB Enzymes GmbH (Darmstadt, Germany)
  • BBSRC IPA Award BB/M022978/1 (30/09/2015 - 29/09/2018) - Mapping the Catalytic Landscape of a Novel Phytase (Hemmings PI, Brearley Co-I)
  • BBSRC LINK Award BB/N002024/1 (27/06/2016 - 26/11/2018) - Phytases beyond phosphate, how inositol improves feed conversion ratio in poulty (Brearley PI, Hajihosseini Co-I)