Career History

  • 1970 BSc "Genetics", University of Edinburgh
  • 1973 PhD University of Edinburgh
  • 1973 – 1989 Research Scientist at John Innes Institute, Norwich, UK
  • 1989 – present Professor Biological Sciences, University of East Anglia, Norwich, UK
     

ResearcherID 
http://www.researcherid.com/rid/D-1859-2009

All Publications

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Johnston, A., Green, R., Todd, J.

(2016)

Enzymatic breakage of dimethylsulfoniopropionate - a signature molecule for life at sea

in Current Opinion in Chemical Biology

31.

pp. 58-65

Full Text UEA Repository

(Article)


Sun, J., Todd, J. D., Thrash, J. C., Qian, Y., Qian, M. C., Temperton, B., Guo, J., Fowler, E. K., Aldrich, J. T., Nicora, C. D., Lipton, M. S., Smith, R. D., De Leenheer, P., Payne, S. H., Johnston, A. W., Davie-Martin, C. L., Halsey, K. H., Giovannoni, S. J.

(2016)

The abundant marine bacterium Pelagibacter simultaneously catabolizes dimethylsulfoniopropionate to the gases dimethyl sulfide and methanethiol

in Nature Microbiology

1.

Full Text UEA Repository

(Article)


Curson, A., Burns, O., Voget, S., Daniel, R., Todd, J., McInnis, K., Wexler, M., Johnston, A.

(2014)

Screening of Metagenomic and Genomic Libraries Reveals Three Classes of Bacterial Enzymes That Overcome the Toxicity of Acrylate

UEA Repository

(Paper)


Curson, A. R. J., Burns, O. J., Voget, S., Daniel, R., Todd, J. D., McInnis, K., Wexler, M., Johnston, A. W. B.

(2014)

Screening of Metagenomic and Genomic Libraries Reveals Three Classes of Bacterial Enzymes That Overcome the Toxicity of Acrylate

in PLoS ONE

9.

Full Text UEA Repository

(Article)


Wischer, D., Kumaresan, D., Johnston, A., El Khawand, M., Stephenson, J., Hillebrand-Voiculescu, A., Chen, Y., Murrell, C.

(2014)

Identification and characterization of active methylamine degrading bacteria in Movile Cave

in The ISME Journal

9.

pp. 195-206

UEA Repository

(Article)


Green, R., Todd, J., Johnston, A.

(2013)

Manganese uptake in marine bacteria; the novel MntX transporter is widespread in Roseobacters, Vibrios, Alteromonadales and the SAR11 and SAR116 clades

in The ISME Journal

7.

pp. 581-591

Full Text UEA Repository

(Article)


Todd, J., Kirkwood, M., Newton-Payne, S., Johnston, A.

(2012)

DddW, a third DMSP lyase in a model Roseobacter marine bacterium, Ruegeria pomeroyi DSS-3

in The ISME Journal

6.

pp. 223-226

Full Text UEA Repository

(Article)


Lucas-Elio, P., Goodwin, L., Woyke, T., Pitluck, S., Nolan, M., Kyrpides, N., Detter, J., Copeland, A., Teshima, H., Bruce, D., Detter, C., Tapia, R., Han, S., Land, M., Ivanova, M., Mikhailova, N., Johnston, A., Sanchez-Amat, A.

(2012)

Complete genome sequence of the melanogenic marine bacterium Marinomonas mediterranea type strain (MMB-1T)

in Standards in Genomic Sciences

6.

UEA Repository

(Article)


Curson, A., Fowler, E., Dickens, S., Johnston, A., Todd, J.

(2012)

Multiple DMSP lyases in the γ-proteobacterium Oceanimonas doudoroffii

in Biogeochemistry

110.

pp. 109-119

Full Text UEA Repository

(Article)


Lucas-Elío, P., Goodwin, L., Woyke, T., Pitluck, S., Nolan, M., Kyrpides, N., Detter, J., Copeland, A., Lu, M., Bruce, D., Detter, C., Tapia, R., Han, S., Land, M., Ivanova, N., Mikhailova, N., Johnston, A., Sanchez-Amat, A.

(2012)

Complete genome sequence of Marinomonas posidonica type strain (IVIA-Po-181T)

in Standards in Genomic Sciences

7.

pp. 31-43

Full Text UEA Repository

(Article)


Sun, L., Curson, A., Todd, J., Johnston, A.

(2012)

Diversity of DMSP transport in marine bacteria, revealed by genetic analyses

in Biogeochemistry

110.

pp. 121-130

Full Text UEA Repository

(Article)


Todd, J., Curson, A., Sullivan, M., Kirkwood, M., Johnston, A.

(2012)

The Ruegeria pomeroyi acuI Gene Has a Role in DMSP Catabolism and Resembles yhdH of E. coli and Other Bacteria in Conferring Resistance to Acrylate

in PLoS ONE

7.

Full Text UEA Repository

(Article)


Curson, A., Todd, J., Sullivan, M., Johnston, A.

(2011)

Catabolism of dimethylsulphoniopropionate: microorganisms, enzymes and genes

in Nature Reviews Microbiology

9.

pp. 849-859

Full Text UEA Repository

(Article)


White, G., Singleton, C., Todd, J., Cheesman, M., Johnston, A., Le Brun, N.

(2011)

Heme binding to the second, lower-affinity site of the global iron regulator Irr from Rhizobium leguminosarum promotes oligomerization

in FEBS Journal

278.

pp. 2011-2021

Full Text UEA Repository

(Article)


Todd, J., Curson, A., Kirkwood, M., Sullivan, M., Green, R., Johnston, A.

(2011)

DddQ, a novel, cupin-containing, dimethylsulfoniopropionate lyase in marine roseobacters and in uncultured marine bacteria

in Environmental Microbiology

13.

pp. 427-438

Full Text UEA Repository

(Article)


Sullivan, M., Curson, A., Shearer, N., Todd, J., Green, R., Johnston, A.

(2011)

Unusual regulation of a leaderless operon involved in the catabolism of dimethylsulfoniopropionate in Rhodobacter sphaeroides

in PLoS ONE

6.

Full Text UEA Repository

(Article)


Curson, A., Sullivan, M., Todd, J., Johnston, A.

(2011)

DddY, a periplasmic dimethylsulfoniopropionate lyase found in taxonomically diverse species of Proteobacteria

in The ISME Journal

5.

pp. 1191-1200

Full Text UEA Repository

(Article)


Wexler, M., Bond, P., Richardson, D., Johnston, A.

(2011)

Functional screening of a wide host-range metagenomic library from a wastewater treatment plant yields a novel alcohol/aldehyde dehydrogease

Wiley-Blackwell

ISBN 978-0-470-64719-6

UEA Repository

(Chapter)


Singleton, C., White, G., Todd, J., Marritt, S., Cheesman, M., Johnston, A., Le Brun, N.

(2010)

Heme-responsive DNA binding by the global iron regulator Irr from Rhizobium leguminosarum

in The Journal of Biological Chemistry

285.

pp. 16023-16031

Full Text UEA Repository

(Article)


Kirkwood, M., Le Brun, N., Todd, J., Johnston, A.

(2010)

The dddP gene of Roseovarius nubinhibens encodes a novel lyase that cleaves dimethylsulfoniopropionate into acrylate plus dimethyl sulfide

in Microbiology

156.

pp. 1900-1906

Full Text UEA Repository

(Article)


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Key Research Interests

I am a microbial geneticist, with interests in the ways that bacteria undertake biotransformations that relate to biogeochemical cycling and in the ways that these organisms regulate these processes in response to external factors. 

Current Research Projects

  • Identification of the ways in which bacteria catabolise the anti-stress molecule dimethylsulfoniopropionate that is made by many marine phytoplankton and whose breakdown by microbes represents a major step in the global sulfur cycle.
  • Analysis of metal-responsive gene regulation in Rhizobium and in marine alpha-proteobacteria.

Life in our research group

We have used genetics set to determine how microbes catabolise dimethylsulfoniopropionate (DMSP), a hugely abundant anti-stress molecule made by marine plankton, some seaweeds and a very few land plants. These studies identified a remarkable array of totally different ways in which different lineages of microbes break down DMSP. Some of these release the gaseous by-product dimethyl sulfide (DMS) which itself is environmentally influential – it is a potent chemo-attractant for marine animals and its oxidation products are “cloud condensation nuclei” that initiate cloud formation over the oceans. The “ddd” genes that are involved in DMS production are prone to rampant horizontal gene transfer, and have been transferred, at least once, between marine bacteria and various fungi. Their regulation also has interesting and unusual features. By examining the distribution of the ddd genes in individual genomes and in metagenomic data sets, we can deduce their abundance in different microbial lineages and in different environments, with implications on the eco-physiology of this important step in the global sulfur cycle. Our field work takes us to the far north of Norfolk, where we sample the salt marshes, the beaches and the occasional fish shop for interesting bacteria and fungi. 

Although transition metals such as iron and manganese are important in the nutrition of bacteria in the seas, little is known of the ways in these are acquired or the ways in which the uptake genes are regulated. In a new programme, we use genetics and genomics to address these topics. This builds on earlier studies on the iron-regulon of the Rhizobia a group of bacteria that induce nitrogen-fixing nodules on legume plants. These bacteria use two global regulators, called Irr and RirA, which are very different from the conventional Fur global regulator, which represses many genes in many other bacteria, including the model species Bacillus subtilis and Escherichia coli. Working in concert, Irr is a heme-binding polypeptide that represses many genes under conditions of iron shortage - in contrast, the FeS-containing regulator RirA represses a different suite of genes under conditions where iron is in short supply.
Our work is much aided by productive collaborations with colleagues in Norwich (Nick Le Brun, Charles Brearley, Tony Davy, Phil Page, Phil Poole, Arnoud van Vliet, Yohan Chan, Allan Downie and Paul Nicholson) and elsewhere (such as Chris Dupont, Krystal Rypien, Michael Steinke, Dmitry Rodionov, Steve Giovanonni, Colin Murrell and Hendrik Schaefer).

PhD Positions

Click here for current PhD opportunities in Biological Sciences. But feel free to email me to discuss projects outside these areas and alternative sources of funding.

Postdocs & fellows

  • Dr Andrew RJ Curson
  • Dr Jonathan D Todd (part-time)
  • Mark Kirkwood (PhD Student)
  • Emily Fowler (PhD student)
  • Rob Green (PhD Student, with Arnoud van Vliet, Institute of Food Research)

 

External Activities and Indicators of Esteem

  • Co-organizer Society General Microbiology Meeting on Metagenomics, April 2006.
  • Member Steering Committee European Nitrogen Fixation Conference
  • Member of NERC Peer Review College

Administrative Posts

  • Module organiser for Year 2 module “Microbiology” (2B28)
  • Associate Dean Research in the Science Faculty at UEA
  • Member of the University Honorary Degrees Committee
  • Member of the University Senate Disciplinary Committee