Dr Samuel Fountain
| Job Title | Contact | Location |
|---|---|---|
| Lecturer in Pharmacology |
S dot J dot Fountain at uea dot ac dot uk
Tel: +44 (0)1603 59 7326 |
Biology 1.23 |
Career
- BBSRC David Phillips Fellow & Lecturer, University of East Anglia (2010 – present)
- BBSRC David Phillips Fellow & Lecturer, University of Leeds (2008 – 2010)
- Wellcome Trust Research Associate, University of Manchester (2004 – 2008)
- MRC-funded PhD, University of Leeds (2000 – 2004)
- 1st Class BSc (Hons) Pharmacology, University of Leeds (1997 – 2000)
ResearcherID
http://www.researcherid.com/rid/C-2142-2011Key Research Interests
Current Research Projects
- Intracellular P2X receptors as regulators of acidic calcium stores
- P2 receptor function and regulation in cardiovascular disease
- P2 receptors in human pathogens, novel therapeutic targets
- Molecular evolution of ATP signalling
- Dictyostelium discoideum as a model system to study ATP signalling
Life in our research group
The laboratory is extremely well equipped thanks to generous funding by the Biotechnology & Biological Sciences Research Council (BBSRC). Many techniques are available to investigate cell-to-cell and intracellular communication by signalling nucleotides, ranging from function at the cellular level to molecular interactions and genetics. Functional techniques used in the lab include patch-clamp electrophysiology, cellular calcium imaging and fluorescent time-lapse microscopy; molecular and biochemical techniques include molecular cloning and mutagenesis, gene knockdown, forward and reverse genetics in Dictyostelium, expression of recombinant receptors and ion channels, subcellular fractionation and protein-protein interaction. We are a lively and dynamic group, regularly presenting our work at national and international meetings.PhD Positions
Please email me to discuss PhD opportunities and projects within the School of Biological Sciences.Article
Sivaramakrishnan, V and Fountain, SJ (2013) Intracellular P2X receptors as novel calcium release channels and modulators of osmoregulation in Dictyostelium: A comparison of two common laboratory strains. Channels, 7 (1). ISSN 1933-6950
Li, J and Fountain, SJ (2012) Fluvastatin suppresses native and recombinant human P2X4 receptor function. Purinergic Signalling (Epub ahead of print). ISSN 1573-9538
Sivaramakrishnan, V, Bidula, S, Katikaneni, D and Fountain, SJ (2012) Constitutive lysosome exocytosis releases ATP and engages P2Y receptors in human monocytes. The Journal of Biological Chemistry, 125. pp. 4567-4575.
Sivaramakrishnan, V and Fountain, SJ (2012) A Mechanism of Intracellular P2X Receptor Activation. Journal of Biological Chemistry, 287 (34). pp. 28315-28326. ISSN 0021-9258
Cheong, Alex, Fountain, Samuel J. and Beech, David J. (2009) Quantitative RT-PCR Methods for Investigation of Low Copy Potassium Channel Gene Expression in Native Murine Arteries. Potassium Channels, 491 (I). pp. 19-33.
Fountain, Samuel J. and Burnstock, Geoffrey (2009) An evolutionary history of P2X receptor. Purinergic Signalling, 5 (3). pp. 269-272.
Young, MT, Fisher, JA, Fountain, SJ, Ford , RC, North, AR and Khakh, BS (2008) Molecular Shape, Architecture, and Size of P<sub>2</sub>X<sub>4</sub> Receptors Determined Using Fluorescence Resonance Energy Transfer and Electron Microscopy. The Journal of Biological Chemistry, 283 (38). pp. 26241-26251.
Fountain, SJ, Cao, L, Young, MT and North, AR (2008) Permeation Properties of a P2X Receptor in the Green Algae Ostreococcus tauri The Journal of Biological Chemistry, 283. pp. 15122-15126.
Cao, Lishuang, Young, Mark T., Broomhead, Helen E., Fountain, Samuel J. and North, Alan R. (2007) Thr339-to-Serine Substitution in Rat P2X2 Receptor Second Transmembrane Domain Causes Constitutive Opening and Indicates a Gating Role for Lys308 The Journal of Neuroscience, 27 (47). pp. 12916-12923.
Fountain, Samuel J., Cheong, Alex, Li, Jing, Dondas, Naciye Y., Zeng, Fanning, Wood, Ian C. and Beech , David J. (2007) Kv1.5 potassium channel gene regulation by Sp1 transcription factor and oxidative stress AJP - Heart and Circulatory Physiology, 293 (5). pp. 2719-2725.
Fountain, Samuel J., Parkinson, Katie, Young, Mark T., Cao, Lishuang, Thompson, Christopher R. L. and North, Alan R. (2007) An intracellular P2X receptor required for osmoregulation in Dictyostelium discoideum. Nature, 448. pp. 200-203.
Fountain, Samuel J. and North, Alan R. (2006) A C-terminal Lysine That Controls Human P2X4 Receptor Desensitization The Journal of Biological Chemistry, 281. pp. 15044-15049.
Zeng, Fanning, Xu, Shang-Zhong, Jackson, Philippa K., McHugh, Damian, Kumar , Bhaskar, Fountain, Samuel J. and Beech, David R. (2004) Human TRPC5 channel activated by a multiplicity of signals in a single cell The Journal of Physiology, 559. pp. 739-750.
Fountain, Samuel J., Cheong, A., Flemming, R., Mair, L., Sivaprasadarao, A. and Beech, D. J. (2004) Functional up-regulation of KCNA gene family expression in murine mesenteric resistance artery smooth muscle. The Journal of Physiology, 556. pp. 29-42.
Fountain, Samuel J. Neurotransmitter Receptor Homologues of Dictyostelium discoideum. Journal of Molecular Neuroscience, 41 (2). pp. 263-266.
External Activities and Indicators of Esteem
- Research Grant Reviewer for BBSRC and The Wellcome Trust
- The Physiological Society, London (Member)
- The British Society for Cell Biology (Member)
- BBSRC David Phillips Fellow
- Purinergic Signalling (Editor)
- The Physiological Society (Mentor)
- The British Pharmacological Society (Member)
